Minigraph-Cactus, a method to efficiently combine multiple reference genome assemblies into a pangenome reference graph, can be used to improve accuracy of read mapping and variant calling compared with a single reference in downstream applications.
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This is a summary of: Hickey, G. et al. Pangenome graph construction from genome alignments with Minigraph-Cactus. Nat. Biotechnol. https://doi.org/10.1038/s41587-023-01793-w (2023).
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Combining reference genomes into a pangenome graph improves accuracy and reduces bias. Nat Biotechnol (2023). https://doi.org/10.1038/s41587-023-01828-2