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Combining reference genomes into a pangenome graph improves accuracy and reduces bias

Minigraph-Cactus, a method to efficiently combine multiple reference genome assemblies into a pangenome reference graph, can be used to improve accuracy of read mapping and variant calling compared with a single reference in downstream applications.

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Fig. 1: Minigraph-Cactus pangenome construction.


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This is a summary of: Hickey, G. et al. Pangenome graph construction from genome alignments with Minigraph-Cactus. Nat. Biotechnol. (2023).

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Combining reference genomes into a pangenome graph improves accuracy and reduces bias. Nat Biotechnol 42, 580–581 (2024).

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