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Measuring the impact of chromatin context on transcription factor binding affinities

We have designed a method, binding affinities to native chromatin by sequencing (BANC-seq), to determine the transcription factor concentrations required for binding to regulatory elements across the genome. Our study shows that chromatin context and DNA accessibility are key regulators of transcription factor binding.

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Fig. 1: Overview of the BANC-seq experimental procedure and interpretation.


  1. ENCODE Project Consortium. An integrated encyclopedia of DNA elements in the human genome. Nature 489, 57–74 (2012). An important paper that gives an overview of TF binding across the genome and epigenome.

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This is a summary of: Neikes, H. K. et al. Quantification of absolute transcription factor binding affinities in the native chromatin context using BANC-seq. Nat. Biotechnol. (2023).

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Measuring the impact of chromatin context on transcription factor binding affinities. Nat Biotechnol (2023).

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