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Engineered upstream open reading frames predictably downregulate mRNA translation in plants

Fine-tuning gene expression is crucial for generating quantitative phenotypic changes and crop improvement. Using CRISPR–Cas base editing and prime editing, we engineered upstream open reading frames — eukaryotic translational control elements — in rice to incrementally downregulate translation to predictable and desired levels.

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Fig. 1: Breeding plants with a predicted phenotype by engineering uORFs.


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This is a summary of: Xue, C. et al. Tuning plant phenotypes by precise, graded downregulation of gene expression. Nat. Biotechnol. (2023).

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Engineered upstream open reading frames predictably downregulate mRNA translation in plants. Nat Biotechnol (2023).

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