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CRISPR-Csm for eukaryotic RNA knockdown and imaging without toxicity

Targeted RNA degradation remains a challenge using existing methods owing to off-target effects and toxicity. We adapted the CRISPR-Csm complex, a multi-protein effector from type III CRISPR systems, for precise knockdown of nuclear or cytoplasmic transcripts in eukaryotic cells with minimal side effects.

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Fig. 1: Overview of the CRISPR-Csm system.


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This is a summary of: Colognori, D., Trinidad, M. & Doudna, J. A. Precise transcript targeting by CRISPR-Csm complexes. Nat. Biotechnol. (2023).

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CRISPR-Csm for eukaryotic RNA knockdown and imaging without toxicity. Nat Biotechnol 41, 1204–1205 (2023).

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