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CLASH enables large-scale parallel knock-in for cell engineering

Nature Biotechnology volume 41, pages 1202–1203 (2023)Cite this article

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CLASH is a CRISPR-based platform that enables the parallel knock-in via homology-directed repair of a large pool of transgene variants encoded in adeno-associated virus vectors. CLASH can be applied to the systematic and unbiased selection of favorable features in T cells and, in principle, other cell types.

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Fig. 1: Development of CLASH system for massively parallel knock-in engineering and selection in primary human T cells.

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This is a summary of: Dai, X. et al. Massively parallel knock-in engineering of human T cells. Nat. Biotechnol. https://doi.org/10.1038/s41587-022-01639-x (2023).

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CLASH enables large-scale parallel knock-in for cell engineering. Nat Biotechnol 41, 1202–1203 (2023). https://doi.org/10.1038/s41587-023-01664-4

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