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Protein identification using a digestion-free nanopore approach

Sequencing of proteins is a technically difficult task that typically requires digestion into short peptides before detection and identification. We developed a digestion-free method to chemically unfold and ‘scan’ full-length proteins through a nanopore, producing electrical fingerprints unique to individual protein molecules that are useful in their identification.

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Fig. 1: Full-length protein transport through a nanopore.


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This is a summary of: Yu, L. et al. Unidirectional single-file transport of full-length proteins through a nanopore. Nat. Biotechnol. (2023)

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Protein identification using a digestion-free nanopore approach. Nat Biotechnol 41, 1078–1079 (2023).

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