Sequencing of proteins is a technically difficult task that typically requires digestion into short peptides before detection and identification. We developed a digestion-free method to chemically unfold and ‘scan’ full-length proteins through a nanopore, producing electrical fingerprints unique to individual protein molecules that are useful in their identification.
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Alfaro, J. A. et al. The emerging landscape of single-molecule protein sequencing technologies. Nat. Methods 18, 604–617 (2021). This review article presents the development of protein sequencing technologies.
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This is a summary of: Yu, L. et al. Unidirectional single-file transport of full-length proteins through a nanopore. Nat. Biotechnol. https://doi.org/10.1038/s41587-022-01598-3 (2023)
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Protein identification using a digestion-free nanopore approach. Nat Biotechnol (2023). https://doi.org/10.1038/s41587-022-01599-2