Nanobody–Tn5 transposase fusion proteins enable detection of two histone marks and open chromatin regions at the same time, with single-cell resolution.
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References
Preissl, S., Gaulton, K. J. & Ren, B. Characterizing cis-regulatory elements using single-cell epigenomics. Nat. Rev. Genet. https://doi.org/10.1038/s41576-022-00509-1 (2022). This review article presents an overview of single-cell epigenomic technologies.
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Stuart, T. et al. Nanobody-tethered transposition allows for multifactorial chromatin profiling at single-cell resolution. Nat. Biotechnol. https://doi.org/10.1038/s41587-022-01588-5 (2022). This paper, together with our study, introduces nanobody–Tn5 transposase fusion proteins for single-cell and multimodal profiling of histone modifications.
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This is a summary of: Bartosovic, M. & Castelo-Branco, G. Multimodal chromatin profiling using nanobody-based single-cell CUT&Tag. Nat. Biotechnol. https://doi.org/10.1038/s41587-022-01535-4 (2022).
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Single-cell nanobody-based profiles of multiple epigenetic modalities and chromatin velocity. Nat Biotechnol 41, 767–768 (2023). https://doi.org/10.1038/s41587-022-01596-5
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DOI: https://doi.org/10.1038/s41587-022-01596-5
