Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Multiplexed mapping of chromatin features at single-cell resolution

Single-cell nanobody-tethered transposition followed by sequencing (scNTT-seq) is a new assay that measures the genome-wide presence of multiple histone modifications and protein–DNA binding sites at single-cell resolution. scNTT-seq generates high-resolution multimodal maps of chromatin states with high accuracy and sensitivity.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Fig. 1: scNTT-seq provides accurate single-cell multimodal chromatin profiles.

References

  1. Kaya-Okur, H. S. et al. CUT&Tag for efficient epigenomic profiling of small samples and single cells. Nat. Commun. 10, 1930 (2019). This article introduced Tn5 immuno-tethering as a tool for profiling chromatin features.

    Article  PubMed  PubMed Central  Google Scholar 

  2. Zhang, B. et al. Characterizing cellular heterogeneity in chromatin state with scCUT&Tag-pro. Nat. Biotechnol. 40, 1220–1230 (2022). This article extends the original single-cell CUT&Tag protocol to include surface protein readout.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Stuart, T. et al. Comprehensive integration of single-cell data. Cell 177, 1888–1902.e21 (2019). This article introduced the concept of single-cell epigenetics data integration.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Stoeckius, M. et al. Simultaneous epitope and transcriptome measurement in single cells. Nat. Methods 14, 865–868 (2017). This article used oligonucleotide-conjugated antibodies to profile RNA and surface proteins at the single-cell level.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Mimitou, E. P. et al. Scalable, multimodal profiling of chromatin accessibility, gene expression and protein levels in single cells. Nat. Biotechnol. 39, 1246–1258 (2021). This article used oligonucleotide-conjugated antibodies to profile DNA accessibility and surface proteins at the single-cell level.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Additional information

Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

This is a summary of: Stuart, T. et al. Nanobody-tethered transposition enables multifactorial chromatin profiling at single-cell resolution. Nat. Biotechnol. https://doi.org/10.1038/s41587-022-01588-5 (2022).

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Multiplexed mapping of chromatin features at single-cell resolution. Nat Biotechnol 41, 769–770 (2023). https://doi.org/10.1038/s41587-022-01589-4

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41587-022-01589-4

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing