Single-cell nanobody-tethered transposition followed by sequencing (scNTT-seq) is a new assay that measures the genome-wide presence of multiple histone modifications and protein–DNA binding sites at single-cell resolution. scNTT-seq generates high-resolution multimodal maps of chromatin states with high accuracy and sensitivity.
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Kaya-Okur, H. S. et al. CUT&Tag for efficient epigenomic profiling of small samples and single cells. Nat. Commun. 10, 1930 (2019). This article introduced Tn5 immuno-tethering as a tool for profiling chromatin features.
Zhang, B. et al. Characterizing cellular heterogeneity in chromatin state with scCUT&Tag-pro. Nat. Biotechnol. 40, 1220–1230 (2022). This article extends the original single-cell CUT&Tag protocol to include surface protein readout.
Stuart, T. et al. Comprehensive integration of single-cell data. Cell 177, 1888–1902.e21 (2019). This article introduced the concept of single-cell epigenetics data integration.
Stoeckius, M. et al. Simultaneous epitope and transcriptome measurement in single cells. Nat. Methods 14, 865–868 (2017). This article used oligonucleotide-conjugated antibodies to profile RNA and surface proteins at the single-cell level.
Mimitou, E. P. et al. Scalable, multimodal profiling of chromatin accessibility, gene expression and protein levels in single cells. Nat. Biotechnol. 39, 1246–1258 (2021). This article used oligonucleotide-conjugated antibodies to profile DNA accessibility and surface proteins at the single-cell level.
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This is a summary of: Stuart, T. et al. Nanobody-tethered transposition enables multifactorial chromatin profiling at single-cell resolution. Nat. Biotechnol. https://doi.org/10.1038/s41587-022-01588-5 (2022).
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Multiplexed mapping of chromatin features at single-cell resolution. Nat Biotechnol (2022). https://doi.org/10.1038/s41587-022-01589-4