Single-cell nanobody-tethered transposition followed by sequencing (scNTT-seq) is a new assay that measures the genome-wide presence of multiple histone modifications and protein–DNA binding sites at single-cell resolution. scNTT-seq generates high-resolution multimodal maps of chromatin states with high accuracy and sensitivity.
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References
Kaya-Okur, H. S. et al. CUT&Tag for efficient epigenomic profiling of small samples and single cells. Nat. Commun. 10, 1930 (2019). This article introduced Tn5 immuno-tethering as a tool for profiling chromatin features.
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This is a summary of: Stuart, T. et al. Nanobody-tethered transposition enables multifactorial chromatin profiling at single-cell resolution. Nat. Biotechnol. https://doi.org/10.1038/s41587-022-01588-5 (2022).
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Multiplexed mapping of chromatin features at single-cell resolution. Nat Biotechnol 41, 769–770 (2023). https://doi.org/10.1038/s41587-022-01589-4
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DOI: https://doi.org/10.1038/s41587-022-01589-4
