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APPLIED IMMUNOLOGY

CRISPR comes a-knock-in to reprogram antibodies in vivo

Reprogramming of antibody responses in mice is achieved via adeno-associated virus delivery of SaCas9, single guide RNA (sgRNA) and a repair template targeting immunoglobulin genes.

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Fig. 1: In vivo B cell gene-editing mediated by two AAVs.

References

  1. Jeske, A. M., Boucher, P., Curiel, D. T. & Voss, J. E. J. Immunol. 207, 755–764 (2021).

    Article  CAS  Google Scholar 

  2. Mu, Z., Haynes, B. F. & Cain, D. W. Curr. Opin. Virol. 51, 172–178 (2021).

    Article  CAS  Google Scholar 

  3. Nahmad, A. D. et al. Nat. Biotechnol. https://doi.org/10.1038/s41587-022-01328-9 (2022).

  4. Balazs, A. B. et al. Nature 481, 81–84 (2011).

    Article  Google Scholar 

  5. Brinkmann, V. & Heusser, C. H. Cell. Immunol. 152, 323–332 (1993).

    Article  CAS  Google Scholar 

  6. Nahmad, A. D. et al. Nat. Commun. 11, 5851 (2020).

    Article  CAS  Google Scholar 

  7. Huang, D. et al. Nat. Commun. 11, 5820 (2020).

    Article  Google Scholar 

  8. Moffett, H. F. et al. Sci. Immunol. 4, eaax0644 (2019).

    Article  CAS  Google Scholar 

  9. Hartweger, H. et al. J. Exp. Med. 216, 1301–1310 (2019).

    Article  CAS  Google Scholar 

  10. Rurik, J. G. et al. Science 375, 91–96 (2022).

    Article  CAS  Google Scholar 

  11. Gillmore, J. D. et al. N. Engl. J. Med. 385, 493–502 (2021).

    Article  CAS  Google Scholar 

  12. Wardemann, H. et al. Science 301, 1374–1377 (2003).

    Article  CAS  Google Scholar 

  13. Chandler, R. J., Sands, M. S. & Venditti, C. P. Hum. Gene Ther. 28, 314–322 (2017).

    Article  CAS  Google Scholar 

Download references

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Correspondence to Harald Hartweger.

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Competing interests

H.H. and M.C.N. are co-inventors on patent applications relating to B-cell gene-editing (“Antibody gene editing in B lymphocytes,” US patent application numbers 62/877,982, 62/991,482 and 16/938,132; and “Antibody gene editing in B lymphocytes and co-expression of cargo protein,” PCT application no. PCT/US2021/23026). M.C.N. is an inventor on a patent on 3BNC117 (PTC/US2012/038400).

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Hartweger, H., Nussenzweig, M.C. CRISPR comes a-knock-in to reprogram antibodies in vivo. Nat Biotechnol 40, 1183–1184 (2022). https://doi.org/10.1038/s41587-022-01299-x

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