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CRISPR comes a-knock-in to reprogram antibodies in vivo

Reprogramming of antibody responses in mice is achieved via adeno-associated virus delivery of SaCas9, single guide RNA (sgRNA) and a repair template targeting immunoglobulin genes.

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Fig. 1: In vivo B cell gene-editing mediated by two AAVs.


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Correspondence to Harald Hartweger.

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Competing interests

H.H. and M.C.N. are co-inventors on patent applications relating to B-cell gene-editing (“Antibody gene editing in B lymphocytes,” US patent application numbers 62/877,982, 62/991,482 and 16/938,132; and “Antibody gene editing in B lymphocytes and co-expression of cargo protein,” PCT application no. PCT/US2021/23026). M.C.N. is an inventor on a patent on 3BNC117 (PTC/US2012/038400).

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Hartweger, H., Nussenzweig, M.C. CRISPR comes a-knock-in to reprogram antibodies in vivo. Nat Biotechnol 40, 1183–1184 (2022).

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