RNA images recovered by decompression with the segmentation free algorithm (magenta) and directly measured (green) in the same tissue section. White: images overlap exactly. Genes are grouped based on the section in which their direct measurements were made. Insets for all genes in a section show the same region, or an adjacent region if no cells for a given gene were present. Scale bar: 500um. Representative fields of view in each tissue section were chosen such that every gene validated in a tissue section could be visualized in the same region, while quantification of overlap (correlation) was calculated using all cells in a given tissue section, or using randomly selected testing cells (where indicated).