Bioprinted 3D and 4D tissues and organs are expected to revolutionize the biomedical field, eliminating the need for laboratory animals, but little is known about the future impacts of these technologies on drug development.
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References
- 1.
Doke, S. K. & Dhawale, S. C. Saudi Pharm. J. 23, 223–229 (2015).
- 2.
Graham, M. L. & Prescott, M. J. Eur. J. Pharmacol. 759, 19–29 (2015).
- 3.
Cheluvappa, R., Scowen, P. & Eri, R. Pharmacol. Res. Perspect. 5, e00332 (2017).
- 4.
Fleetwood, G. et al. J. Am. Assoc. Lab. Anim. Sci. 54, 192–197 (2015).
- 5.
Vanderburgh, J., Sterling, J. A. & Guelcher, S. A. Ann. Biomed. Eng. 45, 164–179 (2017).
- 6.
Goh, J.-Y. et al. Toxicol. Res. 4, 1297–1307 (2015).
- 7.
Stokes, W. S. Hum. Exp. Toxicol. 34, 1297–1303 (2015).
- 8.
Groeber, F. et al. ALTEX 33, 415–422 (2016).
- 9.
Burden, N. et al. Nano Today 12, 10–13 (2017).
- 10.
Löwa, A., Jevtić, M., Gorreja, F. & Hedtrich, S. Exp. Dermatol. 27, 476–483 (2018).
- 11.
Rosania, K. Lab. Anim. 42, 189–190 (2013).
- 12.
Kim, K., Park, H. & Lim, K.-M. Toxicol. Res. 31, 97–104 (2015).
- 13.
Ford, K. A. ILAR J. 57, 226–233 (2016).
- 14.
Atala, A. & Yoo, J. J. Essentials of 3D Biofabrication and Translation (Elsevier Science, 2015).
- 15.
Peng, W. et al. Acta Biomater. 57, 26–46 (2017).
- 16.
Brown, G. E. & Khetani, S. R. Philos. T. R. Soc. B 373, e2017.0225 (2018).
- 17.
Ahn, S. H. et al. J. Tissue Eng. Regen. Med. 13, 663–676 (2016).
- 18.
Gao, G. & Cui, X. Biotechnol. Lett. 38, 203–211 (2016).
- 19.
Huang, Y. et al. Biotechnol. J. 12, 1600734 (2017).
- 20.
Hunsberger, J. et al. Curr. Stem Cell Rep. 2, 118–127 (2016).
- 21.
Yang, G. H., Yeo, M., Koo, Y. W. & Kim, G. H. Macromol. Biosci. 19, e1800441 (2019).
- 22.
Ashammakhi, N. et al. Biotechnol. J. 13, e1800148 (2018).
- 23.
Mammadov, E. Cyprus J. Med. Sci. 3, 186–188 (2018).
- 24.
Moldovan, N. I. J. Cell. Mol. Med. 22, 2964–2969, https://doi.org/10.1111/jcmm.13598 (2018).
- 25.
Awad, A., Trenfield, S. J., Goyanes, A., Gaisford, S. & Basit, A. W. Drug Discov. Today 23, 1547–1555 (2018).
- 26.
Charbe, N., McCarron, P. A. & Tambuwala, M. M. World J. Clin. Oncol. 8, 21–36 (2017).
- 27.
Mazzocchi, A., Soker, S. & Skardal, A. Appl. Phys. Rev. 6, 11302 (2019).
- 28.
Drăgănescu, D. Farmacia 67, 1–9 (2019).
- 29.
Lerman, M. J. et al. Appl. Phys. Rev. 5, 41109 (2018).
- 30.
Kačarević, Ž. P. et al. Materials (Basel) 11, 2199 (2018).
- 31.
Jang, J., Yi, H.-G. & Cho, D.-W. ACS Biomater. Sci. Eng. 2, 1722–1731 (2016).
- 32.
European Parliament. Regulation (eu) no 575/2013 of the European Parliament and of the Council. OJEU 176, 1–337 (2013).
- 33.
USDA. Animal Welfare Act and Animal Welfare Regulations (USDA, 2017).
- 34.
Frank, J. Struct. Change Econ. Dyn. 16, 557–575 (2005).
- 35.
Herrmann, K. (ed.). Animal Experimentation: Working Towards a Paradigm Change (Brill, 2019).
- 36.
Ngo, T. D. et al. Compos. B Eng. 143, 172–196 (2018).
- 37.
Ma, X. et al. Adv. Drug Deliv. Rev. 132, 235–251 (2018).
- 38.
Bhanu, P. C. H. Transl. Biomed. 7, 4 (2016); https://doi.org/10.2167/2172-0479.100099
- 39.
Peng, W., Unutmaz, D. & Ozbolat, I. T. Trends Biotechnol. 34, 722–732 (2016).
- 40.
Mohammadi, M. H., Obregón, R., Ahadian, S., Ramón-Azcón, J. & Radisic, M. Curr. Pharm. Des. 23, 2991–3004 (2017).
- 41.
Haque, S., Md, S., Whittaker, M. & Kaminskas, L. M. Curr. Pharm. Des. 24, 5072–5080 (2018).
Acknowledgements
The authors thank the Center for Strategic Studies of Oswaldo Cruz Foundation (CEE-Fiocruz) for institutional support and the respondents who participated in this study. This study was supported by research funds from Inova Fiocruz/Fundação Oswaldo Cruz.
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F.M. designed, planned, coordinated and analyzed the work and revised the paper. L.B. reviewed the literature, interpreted results and wrote the paper. L.R. analyzed and evaluated statistical data and graphs and described results. B.C. organized and conducted the survey protocol and revised the final paper. All authors approved the submitted final version.
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Mota, F., Braga, L., Rocha, L. et al. 3D and 4D bioprinted human model patenting and the future of drug development. Nat Biotechnol 38, 689–694 (2020). https://doi.org/10.1038/s41587-020-0540-1
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