Extended Data Fig. 6: PacBio sequencing of cDNA from two mouse fibroblasts. | Nature Biotechnology

Extended Data Fig. 6: PacBio sequencing of cDNA from two mouse fibroblasts.

From: Single-cell RNA counting at allele and isoform resolution using Smart-seq3

Extended Data Fig. 6

(a) Scatter plot showing the observed RNA molecule length with PacBio (x-axis) against the reconstructed RNA molecule with Smart-seq3 (y-axis). Molecules were matched by the sequencing of the same UMI per gene in the respective sequencing method. (b) Detailed information on the Col1a2 gene, for which Smart-seq3 had reconstructed 1,903 bp. The reconstruction was consistent with the 2,267 bp transcript sequenced by PacBio and both shorter than the 5,348 bp full-length Ensemble annotation. The two gaps in the Smart-seq3 reconstructions are shown as red lines. (c) Scatterplots showing the correspondence between observed molecules from PacBio sequencing (x-axis) and the observed molecules from Illumina sequencing from Smartseq3 cDNA libraries from two mouse fibroblast cells. (d) Pie-chart showing the consistency in isoform assignment between the PacBio and Smartseq3 assigned isoforms.

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