Each row shows a tested reaction condition and the number of genes detected in individual HEK293FT cells at 0.5 M raw fastq reads. The numbers of individual cells that contained at least one million sequenced reads per condition are listed on the right. Several earlier versions of Smart-seq2 with elements of Smart-seq3 chemistry are included as “Smart-seq-UMI” in this figure. The exact reaction conditions per row are listed in Supplementary Table 1. The asterisks in the right shows the significance level (*0.05, **0.01, ***0.001, ****< 0.001 and ns for non-significance) when comparing the numbers of genes detected in each condition against the gene detection of Smart-seq2 (bottom row) using Wilcoxon rank sum (two-sided). test. Boxplots denote median and first and third quartiles. Whiskers indicate the most extreme data point within 1.5 lengths of the box.