Supplementary Figure 12: Comparison of species-level read counts across extraction and sequencing approaches. | Nature Biotechnology

Supplementary Figure 12: Comparison of species-level read counts across extraction and sequencing approaches.

From: Complete, closed bacterial genomes from microbiomes using nanopore sequencing

Supplementary Figure 12

Read counts of species detected by the gold standard approach of bead beating and short read sequencing (x-axis) versus read counts of species detected by the present approach incorporating supplemental lytic enzymes (see Methods) (y-axis). On the log-transformed read counts, the two approaches show a Pearson correlation of 0.79 across samples (n=10). In addition, of the 18,462 total cases in which a given species was more than ten-fold enriched in relative abundance in either approach over the other, we found that our approach yielded the higher relative abundance in 95% of cases, suggesting the potential for richer taxonomic sensitivity by our method.

Back to article page