Supplementary Figure 7: Summary of the Perturb-seq experiment. | Nature Biotechnology

Supplementary Figure 7: Summary of the Perturb-seq experiment.

From: Titrating gene expression using libraries of systematically attenuated CRISPR guide RNAs

Supplementary Figure 7

(a) Schematic of Perturb-seq strategy to capture single-cell transcriptomes with matched sgRNA identities. (b) Summary of sequencing and perturbation assignment statistics. (c) Distribution of number of cells captured per perturbation. Median: 122 cells per perturbation; 5th to 95th percentile: 66 – 277 cells per perturbation. n = 19,587 cells. (d, e) Comparison of (d) growth phenotypes (γ) and (e) relative activities measured in the large-scale mismatched sgRNA screen and in the Perturb-seq experiment. Differences are likely due to the different timescales and the different vectors used. n = 128 sgRNAs; r2 = squared Pearson correlation coefficient.

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