Supplementary Fig. 15: Comparison between MiROM and Laser Scanning Confocal Microscopy (LSCM) micrographs of lipid droplets. | Nature Biotechnology

Supplementary Fig. 15: Comparison between MiROM and Laser Scanning Confocal Microscopy (LSCM) micrographs of lipid droplets.

From: Label-free metabolic imaging by mid-infrared optoacoustic microscopy in living cells

Supplementary Fig. 15

Fixed 3T3-L1 adipocytes (incubation day 8) imaged by (a) MiROM and (b) LSCM upon Nile red staining, FOV 150 µm x 150 µm. Both modalities reveal a co-localized pattern (PCC: 0.84, n=22,500) indicating the congruent information content. Missing information in the MiROM image can be explained by the significant lower spatial resolution achieved in the mid-IR range at 3500 nm (2850 cm-1) than the VIS excitation wavelength for confocal microscopy at 510 nm. Several small lipid droplets or lipid droplet clusters observed in the confocal microscopy image, are not resolved as individual structures in MiROM. They are, however, still detected as rather homogenous contrast distribution. Furthermore, in the MiROM image, the cell bodies can be observed due to their lipid content in the cell membrane, whereas the LSCM lacks in revealing the cell membrane.

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