Supplementary Fig. 11: MiROM micrographs of thick frozen tissue sections. | Nature Biotechnology

Supplementary Fig. 11: MiROM micrographs of thick frozen tissue sections.

From: Label-free metabolic imaging by mid-infrared optoacoustic microscopy in living cells

Supplementary Fig. 11

(a) Brightfield image of a transverse whole-body cryosection of a mouse; the lungs and the heart ventricles are clearly identified. (b) Merged lipid (at 2850 cm-1, yellow) and protein (at 1550 cm-1, blue) map produced by MiROM of the same section in a, indicating two ROIs, total imaging time 26 min. (c) (left) Protein-map, (center) lipid-map, and (right) merged lipid/protein map of the zoomed ROI 1, scale bar: 200 µm, total imaging time 54 min. (d) (left) Protein-map, (center) lipid-map, and (right) merged lipid/protein image of the zoomed ROI 2, scale bar: 250 µm, total imaging time 4 min. ROI 1 shows a lipid-containing deposit within a groove-like space of relatively homogeneous protein contrast, most likely part of the lymphatic vascular network. ROI 2 shows sparsely distributed lipid droplets in the left ventricular cardiac muscle. Contrast bars in mV. Images a-d are representative of five independent experiments.

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