(a) Protein map (at 1550 cm-1) of white adipocytes. Here, three ROIs have been marked: blue dashed square for ROI 1, black dashed square for ROI 2, and green dashed square for ROI 3. Each ROI is centered on a single adipocyte. (b-d) Monitoring of proteins during lipolysis for the selected ROIs in g. The white arrow follows regions changing protein contrast during lipolysis. Common time and presence of ISO for each ROI, is indicated above sequence b. The FOV of a is 250 µm x 250 µm. See also Supplementary Videos 3a, 3b. (e,f) Complementary merged lipid (yellow)-protein (blue) map and lipid-only map (grey) of the cells in a-d. Images a-f are representative of three independent experiments.