(a) Monitoring of induced lipolysis in brown (differentiated PreBAT) adipocytes; lipid maps at 2857 cm-1. Two ROIs enclosing individual adipocytes are marked on each sequence, green dashed circle for ROI 1 and red dashed circle for ROI 2. Time and presence of ISO is indicated at the bottom corners of each frame. Overall relative lipid and protein contrast change for (b) white and (c) brown adipocytes. (d) Relative lipid contrast change for ROIs 1 and 2 of the brown adipocytes in a. The red arrow in b-d indicates when ISO was added. (e) Complementary merged lipid (yellow)-protein (blue) map and protein-only map (grey) of cells in main Fig. 1 m, n. (f) Complementary merged lipid (yellow)-protein (blue) map and protein-only map (grey) of cells in a, scale bars: 50 µm. See also Supplementary Videos 1a, 1b and 2a, 2b. Data in a,c,d,f are representative of three independent experiments. Data in b,e are representative of five independent experiments.