Fig. 1: Overview of targeted microbial isolation through reverse genomics. | Nature Biotechnology

Fig. 1: Overview of targeted microbial isolation through reverse genomics.

From: Targeted isolation and cultivation of uncultivated bacteria by reverse genomics

Fig. 1

a, Diagram showing the steps in reverse genomics-enabled microbiology: (1) Identification of membrane protein-encoding genes in SAGs and MAGs, (2) selection of predicted exposed epitopes, (3) antibody production, (4) purification and fluorescent labeling, (5) staining of target cells from microbiome samples and (6) isolation for (7) genomic sequencing or (8) cultivation. b, X-ray structure of penicillin-binding protein from E. coli, with the modeled TM7 glycosyltransferase domain colored blue and the selected epitope region highlighted in yellow–brown. c, Structural model of TM7a CpsC, with the peptide epitope region in purple.

Back to article page