A) β-coefficients for each of the 11 metabolites retained by all 10 LASSO models from an OLS regression model with Shannon diversity as the dependent variable and sex and age included as covariates in the discovery cohort. The cohort was stratified based on defined BMI cutoffs and models were fitted independently for each BMI class. B) Scatter plot of PFOS and Shannon diversity for participants whose BMI is less than 25 (normal weight), and equal to or greater than 35 (Obese II/III) in the discovery cohort. C) Comparison of strengths of correlations for 5α-androstan-3β-17α and PFOS with Shannon diversity across obesity in the discovery and validation cohorts. D) Scatter plot of 5α-androstan-3β-17α and Shannon diversity for participants whose BMI is less than 35, and greater than or equal to 35 (Obese II/III) in the validation cohort. Pearson r and P-Values are shown. Lines shown are y~x regression lines, while the shaded region corresponds to the 95% confidence intervals for the slope of the line. Abbreviations: 5α-androstan-3β-17α: 5α-androstan-3β-17α-diol disulfate; PFOS: perfluorooctanosulfic acid.