(a) Tissue adenylate triphoshate (ATP) and adenylate monophosphate (AMP) ratio. Blue denotes pre-supercooling and green denotes post-supercooling throughout the figure. The symbols of the dot plot overlay correspond to the unique independent biological replicates (n = 5 throughout the figure) and match between Figs. 2 and 3; Supplementary Figs. 2, 4 and 5; and Supplementary Table 2. (b) Tissue adenylate triphosphate (ATP) and adenylate diphosphate (ADP) ratio. (c) Lactate clearance derived from in and outflow measurements. (d) Lactate concentration (top) and pH (below) of the arterial inflow. (e) Perfusate potassium concentrations. (f) Urea concentrations in the perfusate. Stars denote statistical significance (p < 0.05, repeated measures two-way ANOVA followed by the Sidak multiple comparisons test). Boxes: median and IQR. Whiskers: min and max. Error bars of line graphs: mean ± SEM.