The symbols of the dot plot overlay correspond to the unique independent biological replicates (n = 5) and match between Figs. 2 and 3; Supplementary Figs. 2, 4 and 5; and Supplementary Table 2. Left: melting point of the human livers preserved University of Wisconsin hypothermic preservation (HP) solution. Right: melting point of the same human livers preserved in our supercooling (SC) preservation solution. Star denotes statistical significance (p = 0.001, paired two-tailed student’s t tests).