Supplementary Figure 8: Homopolymer analysis. | Nature Biotechnology

Supplementary Figure 8: Homopolymer analysis.

From: Large dataset enables prediction of repair after CRISPR–Cas9 editing in primary T cells

Supplementary Figure 8

(a) Average fraction of indel mutant reads with deletion in target sites grouped by their homopolymer types. HomP(A) corresponds to target sites that have at least two consecutive A nucleotides adjacent to the cut site, and similarly for HomP(C), HomP(G), and HomP(T). No HomP indicates the rest of the target sites without homopolymers. We show the results for T cells (top, 1,521 sites) and the aggregate results for HEK293, K562 and HCT116 (bottom, 96 sites). The mean and standard error of the mean are shown. (b) SPROUT was evaluated with MMEJ and non-MMEJ deletions, and the accuracies are reported here. On T cells we observe an improved prediction accuracy for non-MMEJ model. We also see a better generalization performance of MMEJ model compared to non-MMEJ model in cancer cell lines. Bootstrap mean and standard deviation is shown in the table.

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