Supplementary Fig. 6: Predicted average minimum distance between original or new C termini and the ssDNA substrate for base editing. | Nature Biotechnology

Supplementary Fig. 6: Predicted average minimum distance between original or new C termini and the ssDNA substrate for base editing.

From: Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors

Supplementary Fig. 6

(a) Crystal structure of the SpCas9:gRNA:DNA ternary complex with the ssDNA bubble partially resolved (PDB: 5F9R)36. Novel CP termini are represented as spheres (original N- and C- termini in dark grey). The minimal linear distance between the predicted position of the novel CP termini (and WT C-terminus) and the furthest resolved nucleobase in the ssDNA bubble (corresponding to protospacer position 12, counting the PAM as positions 21–23) is depicted. (b) Cryo-EM structure of the SpCas9:gRNA:DNA ternary complex with the ssDNA bubble fully resolved (PDB: 5Y36)38. Novel CP termini are represented as spheres (original N- and C- termini in dark grey). The minimal linear distance between the predicted position of the novel CP termini (and WT C-terminus) and a protospacer position typically targeted for base editing (corresponding to protospacer position 4) is depicted. (c) Average of the distances to two different target positions on the ssDNA substrate measured for the novel CP termini (or WT C-terminus) from (a) and (b), listed in ascending order.

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