Supplementary Fig. 2: Peak editing position and indel frequencies for SaABEmax and SaKKH-ABEmax at six NNGRRT or six NNHRRT PAM sites. | Nature Biotechnology

Supplementary Fig. 2: Peak editing position and indel frequencies for SaABEmax and SaKKH-ABEmax at six NNGRRT or six NNHRRT PAM sites.

From: Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors

Supplementary Fig. 2

(a) Top: Percent of all sequencing reads containing an indel following modification by SaABEmax or SaKKH-ABEmax at six genomic sites containing an NNGRRT PAM. Bottom: Representative sample of the top two allelic outcomes at six genomic sites containing NNGRRT PAMs following modification with SaABEmax (n = 1 shown) in HEK293T cells. The top allelic outcome is the unmodified amplicon, followed by the most common editing outcome being a single A-to-G conversion (4 out of 6 sites) within a shifted window (protospacer positions 7–11) (b) Top: Percent of all sequencing reads containing an indel following modification by SaABEmax or SaKKH-ABEmax at six genomic sites containing an NNHRRT PAM (where H = A, C, or T). Bottom: Representative sample of the top two allelic outcomes at six genomic sites containing NNHRRT PAMs following modification with SaKKH-ABEmax (n = 1 shown) in HEK293T cells. The top allelic outcome is the unmodified amplicon, followed by the most common editing outcome being a single A-to-G conversion (5 out of 6 sites) within a shifted window (protospacer positions 7–11). Values and error bars reflect the mean ± s.d. of three independent biological replicates performed by different researchers on different days.

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