Supplementary Fig. 1: Indel frequencies for ABE PAM variants at six NGA PAM sites, six NGCG PAM sites or three other PAM sites. | Nature Biotechnology

Supplementary Fig. 1: Indel frequencies for ABE PAM variants at six NGA PAM sites, six NGCG PAM sites or three other PAM sites.

From: Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors

Supplementary Fig. 1

(a) Percent of all sequencing reads containing an indel following modification by VRQR-ABEmax, xABEmax, ABEmax, or NG-ABEmax at six genomic sites containing an NGA PAM in HEK293T cells. (b) Percent of all sequencing reads containing an indel following modification by VRQR-ABEmax, VRER-ABEmax, xABEmax, ABEmax, or NG-ABEmax at six genomic sites containing an NGCG PAM in HEK293T cells. (c) Percent of all sequencing reads containing an indel following modification by VRQR-ABEmax, xABEmax, ABEmax, or NG-ABEmax at three genomic sites (PAM: GAT, CGCC, TGCC) in HEK293T cells. Values and error bars reflect the mean±s.d. of three independent biological replicates performed by different researchers on different days.

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