Supplementary Figure 2: The four batches of freeze-dried Pichia secreted mVHH-IgA produced for formulating the Pichia mVHH-IgA feed contained similar proportions of V2A and V3A. | Nature Biotechnology

Supplementary Figure 2: The four batches of freeze-dried Pichia secreted mVHH-IgA produced for formulating the Pichia mVHH-IgA feed contained similar proportions of V2A and V3A.

From: Yeast-secreted, dried and food-admixed monomeric IgA prevents gastrointestinal infection in a piglet model

Supplementary Figure 2

(a) Immunoblot analysis of 10% w/v resuspended solutions from the four freeze-dried batches, pre-treated with Endo H, shows similar band intensity. The volume loaded in each well corresponds to 1.2 mg of freeze-dried material. (b) ELISA titration curve depicting identical endpoint titers for each of the four batches of Pichia produced mVHH-IgA in binding the immobilised antigen FaeGac. The slope of V2A and V3A curves are different as the KD of the VHH V2 and V3 is quiet different (0.1 µM and 7.7 µM). The analysis was repeated at least twice with similar results.

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