Supplementary Figure 1: Production of mVHH-IgA in soybean seeds and Pichia fermentate. | Nature Biotechnology

Supplementary Figure 1: Production of mVHH-IgA in soybean seeds and Pichia fermentate.

From: Yeast-secreted, dried and food-admixed monomeric IgA prevents gastrointestinal infection in a piglet model

Supplementary Figure 1

(a) Representative immunoblots of V2A produced in Arabidopsis (At-V2A) and soybean (Gm-V2A), in which endoglycosidase T (Endo T) treatment leads to a mobility shift. A proteolytic fragment of < 30 kDa is prominent in both plant species. (b) Pichia-based expression of V2A (Pp-V2A) and V3A (Pp-V3A) depicted in representative immunoblots, in which Endo T treatment shows a marked mobility shift due to removal of glycans. The immunoblots were repeated at least thrice with similar results.