Supplementary Figure 7: Genome assembly in EBOV dilution series and effect of sequencing depth on amount of viral material sequenced. | Nature Biotechnology

Supplementary Figure 7: Genome assembly in EBOV dilution series and effect of sequencing depth on amount of viral material sequenced.

From: Capturing sequence diversity in metagenomes with comprehensive and scalable probe design

Supplementary Figure 7

(a) Percent of viral genome assembled in a dilution series of viral input in two amounts of human RNA background. There are n = 2 technical replicates for each choice of input copies, background amount, and use of capture (n = 1 replicate for the negative control with 0 copies). Each dot indicates percent of genome assembled, from 200,000 reads, in a replicate; line is through the mean of the replicates. Label to the right of each line indicates amount of background material. Assemblies are from read data presented in Fig. 3a. (b) Number of unique viral reads sequenced at increasing sequencing depth, from an input of 103 viral copies in different amounts of background. Horizontal axis gives the number of total reads to which a sample was subsampled. Each line is a technical replicate (n = 2) and shaded regions are 95% pointwise confidence bands calculated across random subsamplings. Dashed vertical line at 200,000 reads denotes the amount of total reads used in (a) and in Fig. 3a. Viral sequencing data generated after capture with VALL saturates more quickly than without capture. (c) Same as (b), but from an input of 104 viral copies.

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