Flowering plants have evolved numerous intraspecific and interspecific prezygotic reproductive barriers to prevent production of unfavourable offspring1. Within a species, self-incompatibility (SI) is a widely utilized mechanism that rejects self-pollen2,3 to avoid inbreeding depression. Interspecific barriers restrain breeding between species and often follow the SI × self-compatible (SC) rule, that is, interspecific pollen is unilaterally incompatible (UI) on SI pistils but unilaterally compatible (UC) on SC pistils1,4,5,6. The molecular mechanisms underlying SI, UI, SC and UC and their interconnections in the Brassicaceae remain unclear. Here we demonstrate that the SI pollen determinant S-locus cysteine-rich protein/S-locus protein 11 (SCR/SP11)2,3 or a signal from UI pollen binds to the SI female determinant S-locus receptor kinase (SRK)2,3, recruits FERONIA (FER)7,8,9 and activates FER-mediated reactive oxygen species production in SI stigmas10,11 to reject incompatible pollen. For compatible responses, diverged pollen coat protein B-class12,13,14 from SC and UC pollen differentially trigger nitric oxide, nitrosate FER to suppress reactive oxygen species in SC stigmas to facilitate pollen growth in an intraspecies-preferential manner, maintaining species integrity. Our results show that SRK and FER integrate mechanisms underlying intraspecific and interspecific barriers and offer paths to achieve distant breeding in Brassicaceae crops.
In flowering plants, prezygotic reproductive barriers may occur at one or more check points before fertilization to prevent production of unfavourable offspring1. In nature, the stigma of an open flower is exposed to pollen from its own species, closely and distantly related interspecies, therefore must respond accordingly. Within a species, SI is widely utilized as a mechanism to avoid inbreeding depression and promote hybrid vigour by rejecting self-pollen and accepting intraspecific cross-compatible (CP) pollen2,3. Between species, interspecific incompatibility maintains species integrity and often follows the SI × SC rule, that is, interspecific pollen is UI on SI pistils but UC on SC pistils1,4,5,6. So far, little is known about the molecular mechanisms regulating these compatibility systems and their interconnections in the Brassicaceae.
In Brassicaceae SI, self-pollen is recognized by the ligand–receptor interaction between the pollen-expressed SCR/SP11 and its receptor, the stigma-expressed, plasma membrane-localized SRK2,3. This activates two downstream positive regulators of SI, the M-locus protein kinase (MLPK)15 and ARM-repeat containing 1 (ARC1) E3 ubiquitin ligase16. How MLPK functions remains unclear2,15,17. ARC1 targets compatible factors such as the exocyst component EXO70A1 for degradation, blocking pollen hydration2,16,18. We discovered that the female fertility regulator FER receptor kinase7,8,9 maintains a stigmatic gate in Arabidopsis thaliana14 and has a dual role in rejecting SI pollen and facilitating SC pollen germination in Brassica rapa10,11 by signalling a rapid elevation of stigmatic reactive oxygen species (ROS) or its decline, respectively.
SRK controls rejection of UI pollen
We first characterized pollen-induced responses during SI, SC, UI or UC pollination in B. rapa and A. thaliana stigmas by depositing pollen from B. rapa, closely related interspecific Brassica oleracea, more distant intergeneric Barbarea vulgaris and A. thaliana19,20 (Fig. 1a). Of particular interest is crosses involving B. vulgaris owing to its high resistance to fungal and insect pathogens21,22, but intergeneric barriers prevent introgression of its desirable traits into Brassica crops.
We used B. rapa var. pekinensis, an economically important vegetable crop also called heading Chinese cabbage, as a representative SI species for pollen-induced responses on the stigma. A hallmark of SI in Brassicaceae is that its strength is developmentally regulated, due to the progressive increase in the amount of SRK accumulation from bud stage to maturity23 (Extended Data Fig. 1a). Compared with extensive CP pollen tube penetration into mature B. rapa stigmas, SI and interspecific pollen were severely inhibited (Fig. 1b and Extended Data Fig. 1b). Bud-stage B. rapa stigmas were comparably well penetrated by both SI and B. oleracea pollen, reflecting the involvement of an SRK-mediated mechanism in UI, mimicking SI. However, they remained impenetrable by B. vulgaris and A. thaliana pollen (Fig. 1b and Extended Data Fig. 1b), and it is likely that even the low level of SRK in bud-stage B. rapa stigmas is enough to reject intergeneric pollen.
Given that the profile of SRK expression aligns with that of ROS accumulation10, we examined whether stigmatic redox status has a crucial role in the UI response. Like the SI-induced stigmatic ROS increase10,11 but contrary to CP-induced ROS decline, UI pollen stimulated notable ROS increase in mature B. rapa stigmas. Bud-stage stigma ROS was reduced by B. oleracea pollen but was increased by B. vulgaris and A. thaliana pollen by 10 min after pollination (Fig. 1c and Extended Data Fig. 1c,d). Furthermore, sequestrating ROS by sodium salicylate (Na-SA)8,24,25 not only broke the barrier for B. oleracea pollen on mature B. rapa stigmas but also the barrier for B. vulgaris pollen on bud-stage stigmas (Extended Data Fig. 1e–g). Together, these results support UI pollen-induced ROS being essential for their rejection on SI stigmas.
Next, we tested whether SRK, in addition to rejecting SI pollen, also underlies the UI response. Treating S46 B. rapa with AS-BrSRK46 (antisense oligodeoxyribonucleotide (AS-ODN) that specifically suppresses BrSRK46)10, allowed the penetration of B. oleracea pollen tubes into mature stigmas and B. vulgaris pollen tubes into bud-stage stigmas, consistent with ROS reduction (Fig. 1d and Extended Data Fig. 2a–d). Moreover, ‘B. rapa fast plant self-compatible (FPsc)’ expresses a transmembrane domain-deleted SRK, hereafter named BrSRKΔTM, failed to respond with SI and UI pollen-triggered ROS increase and showed compromised rejection of SI and UI pollen (Fig. 1e and Extended Data Fig. 2e–j). Together, these results clearly demonstrate that SRK not only controls SI but also the rejection of interspecific and intergeneric pollen.
Furthermore, A. thaliana, a typical SC Brassicaceae with loss-of-function mutations or complete deletions of the SRK and/or SCR genes23,26,27, allowed pollen penetration from A. thaliana, B. rapa, B. oleracea and B. vulgaris, and showed stigmatic ROS reduction after pollination. However, transgenic A. thaliana expressing SP11/SCR, SRK and ARC1 from the A. halleri of S13 (referred to as A. tha-S13 hereafter), which recapitulates SI28, strongly inhibited interspecific pollen and rapidly increased stigmatic ROS (Fig. 1f,g and Extended Data Fig. 2k–m). In addition, B. oleracea S36 pollen was similarly rejected by B. rapa stigmas of several different S-haplotypes (Extended Data Fig. 2n), suggesting that SRK variations do not differ in their function in UI. Together, these results demonstrated unambiguously that SRK and SRK-dependent high stigmatic ROS levels positively correlate with the rejection of UI pollen, similar to the SI response.
UI and SI activate SRK–FER-regulated ROS
Given the role of FER in regulating ROS8,29, we suppressed BrFER1 in B. rapa stigmas by AS-ODN or crossed fer-4 mutant into SI Arabidopsis, and observed effective inhibition of SI and UI pollen-triggered stigmatic ROS increase, and marked SI and UI breakdown (Fig. 2a,b and Extended Data Fig. 3a–g). Suppressing BrANJEA1 (BrANJ1), which complexes with FER14, or BrRBOHF, which produces ROS10, also severely inhibited ROS increase and compromised SI and UI pollen rejection (Extended Data Figs. 3h–l and 4). Furthermore, yeast two-hybrid, bimolecular fluorescent complementation (Extended Data Fig. 5a,b) and protein pull-down assays demonstrated that BrSRK46 interacted with BrFER1 (Fig. 2c,d). Furthermore, protein extracts from SI and UI pollen, and SI determinant BrSCR46, markedly augmented BrSRK46–HA pulled down by MBP–BrFER1 (kinase domain (KD)), whereas CP pollen extracts and BrSCR12 had no effect (Fig. 2c,d and Extended Data Fig. 5c–f). Moreover, when co-expressed in tobacco leaves, BrSCR46 enhanced BrFER1–MYC co-immunoprecipitation with BrSRK46–HA and ROS production in tobacco leaves (Fig. 2e and Extended Data Fig. 5g–i). Together, these results suggest that SCR from SI pollen2,3 and an unknown signal from UI pollen trigger SRK-dependent activation of FER-regulated ROS production by boosting SRK–FER interaction to reject the incompatible pollen.
We then explored how the FER–ROS signalling pathway might connect with known SRK signalling15,16,17,18. Suppressing BrMLPK and BrARC1 by AS-ODN allowed the penetration of SI as well as B. oleracea pollen tubes in mature B. rapa stigmas (Extended Data Fig. 6a–d). However, although AS-BrMLPK inhibited SI and UI-triggered ROS increase, AS-BrARC1 did not (Extended Data Fig. 6e,f). Together, these results suggest that after SRK senses SI and UI pollen, it activates two parallel intracellular signalling pathways: the FER to ROS pathway that mediates pollen rejection, possibly involving MLPK, and the ARC1-mediated pathway for the degradation of compatible factors required for pollen growth.
FER and PCP-Bs favour SC over UC pollen
We used A. thaliana for pollen-induced responses on SC stigmas. Although pollen tubes from B. rapa, B. oleracea or B. vulgaris all penetrated A. thaliana stigmas by 6 h after pollination (HAP) (Fig. 1f), UC pollen tubes were much shorter than SC pollen tubes when examined earlier (1 HAP) (Fig. 3a), suggesting a more stringent barrier for UC pollen. We determined that FER has an important role in suppressing UC pollen in A. thaliana stigma as B. rapa pollen hydrated considerably faster and their tubes were much longer in fer-4 stigmas than those in wild type by 1.5 HAP (Fig. 3b). Moreover, simultaneous deposition of A. thaliana with B. rapa pollen, or A. thaliana with B. oleracea pollen on the same A. thaliana stigma showed a notable reduction in stigmatic barrier strength in fer and rbohd stigmas (Fig. 3c and Extended Data Fig. 7a–c). UC pollen was also slower than SC pollen in inducing ROS decline (Fig. 3d), consistent with FER-mediated ROS functioning as a barrier in SC stigmas to discriminate against interspecific pollen for species integrity.
Pollen coat proteins B-class (PCP-Bs) are highly polymorphic cysteine-rich peptides that are widespread in the Brassicaceae (Extended Data Fig. 7d,e) and are important for pollen hydration12,13,14. In A. thaliana, PCP-Bγ binds to FER and reduces stigmatic ROS to allow pollen growth14. We therefore examined the efficacy of PCP-Bs from different species in suppressing ROS. On A. thaliana stigmas, AtPCP-Bγ induced a marked reduction of ROS as early as 5 min after treatment, but BrPCP-B3 (ref. 12) reduced ROS to comparable levels only by 60 min after treatment. Reciprocally, B. rapa stigma ROS responded to BrPCP-B3 earlier than A. thaliana stigmas (Fig. 3e and Extended Data Fig. 7f,g). Moreover, species-specific PCP-B-induced ROS suppression was recapitulated in Arabidopsis roots, which also express FER29 (Extended Data Fig. 7h,i), thus providing further support. Pull-down assays showed that AtPCP-Bγ competed dose-dependently with BrPCP-B3, but not vice versa, for interaction with the AtFER extracellular domain (ED) (Fig. 3f and Extended Data Fig. 7j). Together, these results suggest that the species-specific match between PCP-Bs and FER is important for a rapid compatibility response and serves as an interspecific barrier to favour intraspecific pollen for fertilization.
Compatible pollen induced-NO reduces ROS
As signalling of ROS and nitric oxide (NO) is intimately engaged30,31, we investigated whether NO regulates stigmatic ROS during SC and UC responses. In A. thaliana stigmas, NO levels increased rapidly, as early as 2 min after pollination with SC pollen, reaching a maximum by 5 min after pollination, and declined sharply to pre-pollination level, paralleling that of pollen hydration (Fig. 4a, upper panel, and Extended Data Fig. 8a,b). However, B. rapa pollen and BrPCP-B3 were notably slower than A. thaliana pollen and AtPCP-Bγ, respectively, in stimulating NO in A. thaliana stigmas (Fig. 4b,c). Furthermore, unlike wild type, fer-4 stigma NO was non-responsive to SC pollination and AtPCP-Bγ treatment (Fig. 4d,e and Extended Data Fig. 8c,d). Species-preferential PCP-B-induced NO increase was also recapitulated in roots (Extended Data Fig. 8e–g). The intraspecific and interspecific pollen and respective PCP-B-induced differential NO increases (Fig. 4b,c) were opposite to their induced changes in ROS (Fig. 3d,e), revealing an inverse functional relationship between NO and ROS in discriminating against interspecific compatible pollen.
Additional observations further supported the role of NO in regulating stigmatic ROS and pollen growth. Scavenging A. thaliana stigma NO by cPTIO31 suppressed SC-induced NO increase and ROS reduction and prevented SC pollen growth (Extended Data Fig. 8h,i). Pollination experiments carried out in NO-deficient32,33,34 and NO-overaccumulating35 mutants similarly supported the importance of NO involvement (Extended Data Fig. 8j–p). Furthermore, in B. rapa, stigmatic NO responded similarly, stimulated by SC but not by SI or UI pollen (Extended Data Fig. 9a,b). Scavenging or increasing NO by cPTIO or S-nitrosoglutathione (GSNO), respectively, also induced opposite changes in ROS and pollen growth in B. rapa stigmas (Extended Data Fig. 9c–h). These results established firmly that NO is specifically induced in stigmas by compatible pollen for ROS reduction and pollen growth.
NO nitrosates FER in compatible response
A major bioactivity of NO is S-nitrosation of specific cysteines in many proteins9,36,37. Given the role of FER and RBOHs examined here (Extended Data Figs. 3 and 4), they could be targets for nitrosation. A tandem mass tag-switch analysis9,38 of GSNO-treated MBP–BrFER1 showed nitrosation of Cys730, and Cys752 in the KD, which are conserved among FERs from many Brassicaceae species (Fig. 4f and Extended Data Fig. 9i–l). AtFER–GFP in transformed A. thaliana stigmas8,29 was also nitrosated after SC pollination (Fig. 4g), suggesting that nitrosation of FER has important roles during the compatible response.
Nitrosation of proteins may affect their stability, biochemical properties and interaction with other proteins9,39. GSNO-treated BrFER1 (KD) and a Cys730Trp (BrFERC730W) conversion, which mimics nitrosation40, both showed compromised interaction with its downstream BrROP2 signalling complex (Fig. 4h and Extended Data Fig. 9m,n). We also observed substantial reduction in AtFER–GFP pulled down by AtROP2 in A. thaliana stigmas with SC pollen (Fig. 4i). Moreover, stigma-expressed RBOHs were also nitrosated in vitro and by pollination (Extended Data Fig. 9o–t), similar to immunity signalling-induced nitrosation36. Together, these results indicate that stigmatic ROS decline is a consequence of compatible pollination-stimulated NO, nitrosating FER to inactivate downstream RAC/ROP-regulated RBOH-dependent ROS production, and the already produced RBOHs to rapidly quell ROS-producing activity in stigmas.
Breaking the barrier for distant breeding
Having established the mechanisms underlying the interspecific barrier at the stigma, we next explored to what extent breaking the barrier might promote distant breeding. We treated B. rapa pistils with Na-SA to reduce the levels of ROS and GSNO to increase NO levels, or AS-ODNs to disrupt the BrSRK–BrFER interaction and BrFER-to-BrRBOH signalling, then pollinated them with SI and UI pollen. By 12 days after pollination, SI B. rapa pollen, B. oleracea pollen and B. vulgaris pollen all resulted in enlarged ovules with developing embryos in these treated pistils (Fig. 5a,b and Extended Data Fig. 10). Additional barriers beyond the stigma must have precluded robust cross-fertilization and hampered hybrid embryo development (Fig. 5a,b and Extended Data Fig. 10). Combining the strategies used here with embryo rescue, an in vitro culture technique widely utilized in distant breeding41, should allow successful development of interspecific hybrid embryos into viable plants.
Upon pollination, pollen and stigma engage in a series of communications to facilitate growth of desirable pollen and discourage invaders and less desirable pollen1. Exploring SI, UI, SC and UC together in one study, we demonstrate how the interplay between two receptor kinases, SRK and FER, provides the capacity to perceive different pollen signals to fine tune the stigmatic redox conditions and determine acceptance or rejection of pollen from intraspecies or sympatric interspecies with similar flowering time. As summarized in our working model (Fig. 5c), we demonstrate how incompatible signals from SI and UI pollen activate FER-mediated ROS production for pollen rejection in SI stigmas, functioning in parallel with ARC1-mediated processes2. We also demonstrate how the species-preferential interaction of PCP-Bs and FER leads to interspecific barriers in SC stigmas.
The Brassicaceae includes many important vegetable and oil crops. Breeding within a species is far from maximizing hybrid vigour owing to the relatively narrow genetic diversity. Breeding between species enriches germplasm resources but is restricted by interspecific barriers, rendering distant breeding with slim chances of success. Achieving interspecific and intergeneric fertilization here by breaking the stigmatic barrier in B. rapa, in particular the production of hybrid embryos with the fungal-resistant and insect-resistant B. vulgaris21,22 (Fig. 5a,b), represents a remarkable feat and major breakthrough, enabling introgression of desirable traits into crops from distant species.
Plant materials and growth conditions
Stigmas from B. rapa var. pekinensis, also known as heading Chinese cabbage, and A. thaliana were mostly used for pollination responses. For interactions on SI stigmas, B. rapa stigmas from a double haploid line of S46 were pollinated with pollen from B. rapa S46 or S12 as SI or CP pollinations, or pollen from B. oleracea (S36), B. vulgaris (SC) and A. thaliana (SC) as UI pollinations. For interactions on SC stigmas, a variety FPsc with a defective allele of SRK that lacks the coding sequence for the transmembrane domain and SC A. thaliana were used. B. rapa stigmas from S46, S12, S9, S40 and S38 were pollinated with B. oleracea (S36) for haplotype-dependent analysis. Transgenic SI A. thaliana28, pAtFER::AtFER–GFP29 plants, homozygous fer-4 (GK-106A06, GABI-Kat)29, srn (the γ-ray mutant)42, hot5-4 (FLAG_298F11, Versailles Genomic Resource Centre)35, noa1 (CS6511)9, rbohd-3 (Salk_070610C)10 and rbohd-4 (CS9555)10 mutants and their corresponding wild-type plants Col-0, C24 or WS have been previously described. Transgenic plants pAtFER::GFP–AtRBOHD were generated by the Agrobacterium tumefaciens-mediated floral dip method43. S13/fer-4 was generated by crossing fer-4 mutant into SI Arabidopsis (S13 of A. halleri); / indicates that these two genetic modifications are in the same Arabidopsis plant.
Seeds of B. rapa, B. oleracea and B. vulgaris were germinated in potted soil (Pindstrup substrate, Denmark). Vernalization was performed in a growth chamber with 10 °C–5 °C, 14 h–10 h light–dark cycles, and light intensity of 100 mmol m−2 s−1. After 1 month of cold treatment for 1-week-old B. rapa, and 3 months for 7–8 leaf stage B. oleracea and B. vulgaris plants, these plants were planted in soil under greenhouse conditions with 25 °C–15 °C, 16 h–8 h light–dark cycles, and light intensity of 300 mmol m−2 s−1. Seeds of A. thaliana and Nicotiana benthamiana were germinated and grew in potted soil in a greenhouse at 22 °C, 16–8 h light–dark cycle with a relative humidity of 60%.
Data involving ROS, NO, pollen hydration and pollen tube length were presented as box plots generated in GraphPad Prism v8.0.1. Unless otherwise indicated, the centre line of box plots denotes the median, the box limits denote the lower and upper quartiles, and the whiskers denote the lowest and highest data points. Protein plots and quantitative PCR with reverse transcription (qRT–PCR) related data were presented as data bars (average ± s.d., n = 3). Data involving changes of stigma NO over time after pollination were presented as a line chart (average ± s.d.; n indicates the number of stigmas). All statistics data were labelled with the exact P value, and dots in data bars and box plots denote individual data points. Each experiment was repeated at least three times with consistent results.
Stigma treatment and pollen growth observation
Compatibility was demonstrated by the number of pollen tubes that had penetrated the stigma papilla cells. Stigma feeding assays followed that of refs. 10,44. B. rapa flowers that have just opened but before anther dehiscence, or bud-stage flowers were emasculated and cut at 3 mm away from the stigmatic surface. Excised stigmas were inserted into basic PGM (5 mM CaCl2, 5 mM KCl, 0.01% H3BO3, 1 mM MgSO4•7H2O, 10% sucrose and 0.8% agarose, pH 7.5) or the treatment medium and kept in a chamber with constant temperature (22.5 °C) and humidity (45%) for the indicated period of time (S-ODNs or AS-ODNs for 1 h; Na-SA, GSNO and cPTIO for 6 h). Treated stigmas were transferred to basic PGM medium and manually pollinated with similar amount of SI, CP or various UI pollen and maintained in the same condition for 6 h or as indicated. Stigmas were then fixed in Canoy’s fixative (ethanol to acetic acid 3:1), softened in 10 M NaOH, and stained in 0.1% aniline blue. Pollen tubes were visualized by epifluorescence (Ex375-328, DM415 and BA351p) on a Nikon Eclipse Ni. Images were captured by a DS-Ri2 digital camera.
For in planta treatment, just open flowers, or bud-stage flowers, on inflorescences of B. rapa plants were treated twice at a 30-min interval, with S-ODN and AS-ODN, Na-SA, GSNO or the corresponding mock solution, supplemented with 0.0125% Tween, then pollinated with a similar amount of SI, CP, B. oleracea or B. vulgaris pollen. The number of enlarged ovules was counted at 12 days after pollination. Embryo clearing and observation followed that of ref. 45 with modifications. Enlarged ovules were cleared in Hoyer’s medium (7.5 g gum arabic, 100 g chloral hydrate, 5 ml glycerol and 60 ml H2O) for 5 days, then observed under differential interference contrast on a Nikon Eclipse Ni microscope equipped with a DS-Ri2 digital camera.
The effect of chemicals or the mutation of stigma-expressed genes on the growth of intraspecific or interspecific pollen on SC A. thaliana stigmas was demonstrated by the rate of pollen hydration or pollen tube length. SC A. thaliana flowers were emasculated at stage 12 (ref. 46), cultured in PGM for 14 h, then pollinated with A. thaliana pollen or B. rapa pollen. For pollen hydration, images were taken at each time point after pollination under differential interference contrast on a Nikon Eclipse Ni microscope equipped with a DS-Ri2 digital camera. The equatorial diameters of pollen grains at various time points were measured in ImageJ v1.53c. For pollen tube length, A. thaliana stigmas with A. thaliana pollen, B. rapa pollen or B. oleracea pollen were processed for aniline blue staining at 1 or 1.5 HAP. In dual-pollination assays, A. thaliana WT and fer-mutant pistils (or other mutant pistils) were simultaneously pollinated with A. thaliana pollen on half and either B. rapa or B. oleracea pollen on the other half of the same stigma, with a clear boundary in between. Pollen growth from each half of the stigma was clearly confined to the corresponding half of the pistils and readily distinguishable.
ODN design and treatment
ODN design and treatment of stigmas followed that of ref. 10. S-ODNs and AS-ODNs were used to target the following genes (accession numbers shown in Supplementary Table 1): BrSRK46, BrFER1 and BrRBOHF. S-ODNs or AS-ODNs were designed based on Sfold (https://sfold.wadsworth.org/cgi-bin/soligo.pl). The BLAST program (https://blast.ncbi.nlm.nih.gov/Blast.cgi) was used to assess potential off-target effect. The ODNs were synthesized in the Beijing Genomics Institution (BGI). Three bases at both 5′ and 3′ end of S-ODN and AS-ODN were phosphorothioate-modified to maintain stability. The sequences of S-ODNs and AS-ODNs were listed in Supplementary Table 2. Stigmas were excised at the style 1 mm away from the top, inserted in PGM containing the S-ODN or AS-ODN and treated for 1 h. Stigmas were subjected to aniline blue assay at 6 HAP to observe pollen growth.
Staining of ROS and NO
For stigmatic ROS staining, B. rapa stigmas or A. thaliana stigmas, unpollinated or at 10 min after pollination with SI, CP or UI pollen, were pretreated in MES buffer (10 mM MES, 5 μM KCl and 50 μM CaCl2, pH 6.15) for 30 min, stained with 50 μM H2DCF-DA (2′,7′-dichlorofluorescein diacetate; Sigma-Aldrich) for 30 min, respectively, then washed at least three times in buffer before observation. For stigmatic NO staining, B. rapa stigmas or A. thaliana stigmas before or after pollination were soaked in Tris buffer (10 mM Tris-HCl and 10 mM KCl, pH 7.5) for 30 min, stained with 20 μM DAF-FM DA (3-amino,4-aminomethyl-2′,7′-difluorescein diacetate; Thermo Scientific) for 1 h, then washed at least three times in buffer before observation.
For treatments, stigmas were first soaked in MES buffer for 30 min, then treated with S-ODNs or AS-ODNs, chemicals, peptides or pollen extracts at indicated concentration and duration in MES buffer supplemented with 0.0125% Tween 20. After washing three times in MES buffer, treated stigmas were stained for ROS or NO as above described.
For root ROS or NO staining, 3-day-old A. thaliana seedlings or 2-day-old B. rapa seedlings were soaked in the corresponding buffer for 30 min, treated with 0.1 μM of AtPCP-Bγ or BrPCP-B3 for the indicated period of time, then stained with 50 μM H2DCF-DA or 20 μM DAF-FM DA for 1 h.
Imaging was carried out under eGFP epifluorescence (Ex470-440, DM4951p and BA525/550), using a Nikon Eclipse Ni and equipped with a DS-Ri2 digital camera. The exposure time for all comparative samples were exactly the same within one experiment. Average ROS or NO signals outlined in the dotted area were measured in Image J v1.53c; ROS or NO in control stigmas were set at 1 for comparative analyses.
For comparison, stigmas were directly stained with the same concentrations of H2DCF-DA or DAF-FM DA for 10 min without buffer pretreatment, and imaged under a confocal microscope (Zeiss LSM880). Changes of ROS and NO were consistent with those imaged under wide-field fluorescence microscope. Wide-field observation, due to its considerably high expedition to sample entire stigma specimens, was used for the massive amount of data gathering required for this study.
For ROS staining of infiltrated tobacco leaves, agrobacterium cells containing BrFER1–MYC, GFP–BrRBOHD2 and BrSRK46–HA were mixed and infiltrated into tobacco leaves. Two days after infiltration, 10 μM GST–BrSCR12 or GST–BrSCR46 were injected into the same leaf and treated for 10 min. Nitro blue tetrazolium (NBT) staining47 was used to detect ROS of the infiltrated leaves. The leaves were vacuum infiltrated with 5 mg ml−1 NBT (in 10 mM sodium citrate, pH 7.0) for 40 min at room temperature. Leaves were then decolourized three times by boiling in decolourized solution (95% ethanol to glycerine 3:1) for 10 min. The images were captured by a digital camera and measured for ROS intensity in Image J v1.53c. For ROS staining of protoplasts, protoplasts were isolated from the above infiltrated tobacco leaves co-expressing BrFER1–MYC, GFP–BrRBOHD2 and BrSRK46–HA, following that of ref. 48. Protoplasts were then treated with buffer, BrSCR12 and BrSCR46, respectively, for 15 min, before staining with 10 μM H2DCF-DA for 10 min. Protoplasts from tobacco leaves infiltrated with buffer were stained for ROS as a control.
Enzymatic activity of RBOHs
Stigmas before or after pollination or treatment were washed three times with the MES buffer and then freezed in liquid N2. Enzymatic activity of RBOHs was measured following the manufacturer’s instructions (Nanjing JC bio). In brief, approximately 0.05 g stigma tissue (approximately 100 stigmas) was ground in liquid N2, extracted in buffer (0.2 M NaH2PO4 and 0.2 M Na2HPO4, pH 7.2) and centrifuged at 4,000g for 20 min at 4 °C. The resulting supernatant was used to measure RBOH activity spectrophotometrically at 340 nm using FAD and NADPH as substrates. The result was shown as the average of three technical replicates of one sample.
RNA isolation and qRT–PCR
Total RNA was extracted via the SteadyPure Universal RNA Extraction Kit (AG21017, Accurate Biotechnology) and reverse transcribed with HiScript III 1st Strand cDNA Synthesis Kit (Vazyme). qRT–PCR was performed on a QuantStudio 3 system (Applied Biosystems) with ChamQ SYBR qPCR Master Mix (Vazyme), using BrACTIN2 as internal controls. A list of gene-specific primers used for qRT–PCR is included in Supplementary Table 3.
For the BrSRK46–HA or BrRBOHD2–GFP construct, sequences encoding the full length of BrSRK46 or BrRBOHD2 were amplified from B. rapa stigma cDNA using 2× Phanta Max Master Mix (Vazyme). The fragment was cloned into the modified pCAMBIA1300 vector with a HA or GFP tag using pEASY-Basic Seamless Cloning and Assembly Kit (TransGen Biotech). For the GST fusion protein constructs, sequences encoding BrPCP-B3 (residues 1–76), BrSCR46 (residues 1–78), BrSCR12 (residues 1–76), full length of BrROP2, C-terminal cytoplasmic region of BrRBOHD1 (CT; residues 758–923), BrRBOHD2 (CT; residues 614–904) and BrRBOHF (CT; residues 767–949) were amplified and cloned into a pGEX-4T-1 vector. For the MBP fusion protein constructs, sequences encoding ED of BrFER1 (residues 14–420), KD of BrFER1 (residues 486–783), mature AtPCP-Bγ (residues 23–76) and AtROP2 were amplified and cloned into a pMAL-p2X vector. For the FLAG fusion protein construct, the ED of AtFER (residues 29–446) and the KD of BrSRK46 (residues 448–860) were amplified and cloned into the pFLAG-CTS vector. For the MYC fusion protein construct, BrFER1 (residues 1–788) was amplified and cloned into pCXSNF. For the bimolecular fluorescent complementation constructs, the KD of BrSRK46 (residues 448–860) and BrFER1 (residues 486–783) were amplified and cloned into pDONR207 by the Gateway BP reaction via Gateway BP Clonase Enzyme Mix (Invitrogen Life Technologies) and cloned into the pEARLY GATE 201 vector or pEARLY GATE 202 by the LR reaction via Gateway LR Clonase Enzyme Mix (Invitrogen Life Technologies). For the yeast two-hybrid constructs, the KD of BrSRK46 (residues 448–860) and the KD of BrFER1 (residues 486–783) were amplified and cloned into the pGADT7 or pGBKT7 vectors. For MBP–BrFER1C730W (KD), the mutant fragment was amplified from the MBP–BrFER1 (KD) vector with appropriate PCR primers using the Fast Mutagenesis System (TransGen Biotech) according to the manufacturer’s instructions. A list of gene-specific primers used for the above constructs is included in Supplementary Table 4.
Protein expression and purification
The constructs of GST, MBP and FLAG fusion protein were transformed into Escherichia coli BL21 (DE3) for protein expression. After induction by 0.5 mM IPTG at 37 °C for 4–6 h, the cells were spun down and resuspended in 5 ml PBS (140 mM NaCl, 2 mM KCl, 2 mM KH2PO4 and 10 mM Na2HPO4.7H2O). Sonicated (SCIENTZ) protein was purified by magnetic GSH beads (BEAVER), amylose magnetic beads (PuriMag Pro) or anti-FLAG magnetic beads (BeyoMag), respectively. The eluted protein was separated by SDS–PAGE and detected by the corresponding antibody after western blot.
For peptide preparation, MBP–AtPCP-Bγ and GST–BrPCP-B3 were expressed in E. coli BL21 cells and purified by corresponding magnetic beads. The AtPCP-Bγ (residues 23–76) peptides were also synthesized by Scilight Biotechnology with more than 95% of purity. The peptides were diluted to 1 mM in sterile ddH2O as stock solution.
For proteins expressed in tobacco leaves, Agrobacterium tumefaciens GV3101 (Weidi) cells containing corresponding constructs were infiltrated into N. benthamiana leaves following standard procedure48. In brief, Agrobacterium cells were spun down at 5,000g for 10 min at 4 °C and resuspended to an optical density at 600 nm of 0.6 in the infiltration buffer (10 mM MES, 10 mM MgCl2 and 0.5% glucose, pH 6.5), with 100 μM acetosyringone added before infiltration. Leaves from 5–6-week-old tobacco plants were infiltrated using a 1-ml syringe. Two days after infiltration, leaves were homogenized in liquid N2, mixed in 1 ml plant protein extraction buffer (75 mM KAc, 300 mM NaCl, 100 mM arginine, 10 mM EDTA, 0.25% Triton X-100, pH 7.4, 1 mM PMSF and 1 mM cocktail protease inhibitor) in a 1.5-ml centrifuge tube and stayed on ice for 20 min. After centrifuge at 13,800g for 10 min at 4 °C, the supernatant was transferred into a new tube for pull-down or co-IP assays.
Protein interaction assays
For the yeast two-hybrid assay of BrFER1 (KD) and BrSRK46 (KD), vector construction, yeast transformation, growth on drop out medium and X-Gal staining followed standard procedure29.
For the bimolecular fluorescent complimentary assay, the pEARLY GATE 201 containing BrFER1 (KD)–nYFP and the pEARLY GATE 202 containing BrSRK46 (KD)–cYFP were transformed into Agrobacterium tumefaciens strain GV3101. Equal volumes of two cultures were mixed and infiltrated into N. benthamiana leaves as described above. Two days after infiltration, imaging was carried out under eGFP epifluorescence (Ex470-440, DM4951p and BA525/550), using a Nikon Eclipse Ni and equipped with a DS-Ri2 digital camera.
For the pull-down assay of BrSRK46–HA by MBP–BrFER1 (KD), BrSRK46–HA protein was extracted from infiltrated tobacco leaves, mixed with amylose magnetic beads (PuriMag Pro)-bound MBP–BrFER1 (KD) bait protein for 6 h at 4 °C. The beads were washed three times in pull-down buffer (50 mM Tris-HCl, pH 7.0, 100 mM NaCl and 0.1% Triton X-100 (v/v)) and boiled in SDS–PAGE loading buffer for 5 min. The proteins were then processed for SDS–PAGE, western blot and immunodetection by anti-MBP antibody (1:15,000; Abmart) or anti-HA antibody (1:5,000; Abmart), and with anti-mouse horseradish peroxidase (HRP)-conjugated secondary antibody (1:15,000; Abmart) followed by the HRP detection kit (Vazyme) analysis with a chemiluminescence imaging system (TIAN NENG). For the effect of peptides, GST–BrSCR46 or GST–BrSCR12, or protein extracts from SI (S46), CP (S12) pollen or interspecific (B. oleracea) pollen on the interaction of BrSRK46–BrFER1 (KD), 0.5 μM peptides or 0.65 mg ml−1 protein extracts were incubated with BrSRK46–HA protein for 1 h before the pull-down assay.
For the co-IP assay of BrFER1–MYC by BrSRK46–HA, GV3101 Agrobacterium cells containing 35S::BrSRK46–HA or 35S::BrFER1–MYC were mixed and infiltrated together into N. benthamiana leaves. Two days after infiltration, 10 μM GST–BrSCR12 or GST–BrSCR46 were infiltrated into the same leaf and treated for 10 min. The leaves were then used for protein extraction. Aliquots of 1 ml supernatant were incubated with 100 μl anti-HA magnetic beads (PuriMag Pro) at 4 °C overnight with rotation. After washing three times, the beads were boiled and the proteins were processed for SDS–PAGE, western bolt and immunodetection by anti-MYC antibody (1:5,000; Abmart) and anti-HA antibody (1:5,000; Abmart) and anti-mouse HRP-conjugated secondary antibody (1:10,000; Abmart).
For the pull-down assay of MBP–BrFER1 (KD) by GST–BrROP2, MBP–BrFER1 (KD) protein was treated with 1, 2 and 5 mM GSNO for 3 h at room temperature, then mixed with GSH bead (BEAVER)-bound, GST–BrROP2 bait protein for 6 h at 4 °C. The beads were boiled and processed for SDS–PAGE, western bolt and immunodetection by anti-MBP antibody (1:15,000; Abmart) or anti-GST antibody (1:5,000; Abmart), and with anti-mouse HRP-conjugated secondary antibody (1:15,000; Abmart). MBP–BrFER1C730W (KD), without GSNO treatment, was processed similarly for the pull-down assay with GST–BrROP2.
For the pull-down of AtFER–GFP by MBP–AtROP2, MBP or MBP–AtROP2 bait proteins were bound with amylose magnetic beads (PuriMag Pro) for 6 h and washed three times with pull-down buffer. A. thaliana stigmas from pAtFER::AtFER–GFP transgenic plants, unpollinated or 10 min after pollination, were used for total protein extraction. AtFER–GFP protein was incubated with corresponding bait protein for 8 h at 4 °C. After washing three times, the beads were boiled and processed for SDS–PAGE, western bolt and immunodetection by anti-GFP antibody (1:10,000; Abmart) or anti-MBP antibody (1:15,000; Abmart), and with anti-mouse HRP-conjugated secondary antibody (1:10,000; Abmart).
For the pull-down assay to examine peptide competition, anti-FLAG magnetic bead (BeyoMag)-bound AtFER (ED)–FLAG bait protein was incubated with GST–BrPCP-B3 with rotation at 4 °C for 2 h, then increasing concentrations of AtPCP-Bγ peptides were added for competition at 4 °C for 3 h. The competition by GST–BrPCP-B3 peptides with MBP–AtPCP-Bγ in interaction with AtFER (ED)–FLAG bait protein was performed similarly. The beads were boiled and processed for SDS–PAGE, western bolt and immunodetection by anti-FLAG antibody (1:5,000; Abmart), anti-GST antibody (1:5,000; Abmart) or anti-MBP antibody (1:15,000; Abmart), and anti-mouse HRP-conjugated secondary antibody (1:5,000; Abmart).
Protein nitrosation assay
In vitro S-nitrosation assay followed that of ref. 35 with modifications, using Pierce S-Nitrosylation Western Blot Kit (90105, Thermo Scientific). Purified proteins of GST–BrRBOHD1 (CT), GST–BrRBOHD2 (CT), GST–BrRBOHF (CT) and MBP–BrFER1 (KD) were desalted by acetone precipitation and resuspended in HENS buffer (100 mM HEPES, pH 7.0, 1 mM EDTA, 0.1 mM neocuproine and 2.5% SDS). Approximately 150 μg proteins per sample were incubated with 1 mM GSNO in a reaction volume of 100 μl HENS buffer for 2 h at room temperature in the dark. The sample was precipitated with cold acetone and resuspended in 100 μl HENS buffer. After incubation at 50 °C for 1 h with 200 mM N-ethylmaleimide (Solarbio) and at room temperature for 30 min, the sample was precipitated with cold acetone and resuspended in HENS buffer. The sample was treated with 60 mM sodium ascorbate and 0.4 mM iodoTMTzero label reagent for 2 h. All the above steps were carried out in the dark. The proteins were finally precipitated by cold acetone and resuspended in 2 M urea buffer. Aliquots of each protein were separated by SDS–PAGE and then analysed by immunoblotting with anti-TMT antibody (1:5,000; Thermo Scientific) and goat anti-mouse IgG (H+L)–HRP (1:10,000; Thermo Scientific) were used for detection of nitrosated protein. In parallel, the proteins were detected for loading with anti-GST antibody (1:5,000; Abmart) or anti-MBP antibody (1:15,000; Abmart), and goat anti-mouse IgG–HRP (1:10,000; Abmart).
For the analysis of S-nitrosation in stigmas before and after pollination, 200 stigmas (approximately 0.1 g) from A. thaliana plants expressing pAtFER::AtFER–GFP or pAtFER::GFP–AtRBOHD, unpollinated or at 10 min after pollination with intraspecific compatible pollen (WT Arabidopsis pollen), were homogenized in liquid N2 and resuspended in 0.7 ml of the plant protein extraction buffer. Protein extracts, 1.5 mg in 150 μl, were precipitated with cold acetone and resuspended in HENS buffer for the detection of nitrosated proteins similar to in vitro nitrosation detection.
Mass spectrometry analysis of nitrosated residue
Mass spectrometric identification of S-nitrosated cysteine residues was carried out by Shanghai Bioprofile Biotechnology (China). The protein pellets were resolved with HENS buffer and N-ethylmaleimide (Sigma) was used to block the free cysteine. The S-nitrosation sites of protein were reduced by the sodium ascorbate (Sigma) specifically and then labelled with iodoTMT zero reagent (Thermo Scientific). The processed proteins were digested with trypsin in 50 mM NH4HCO3 overnight at 37 °C. The peptides were then desalted with C18 cartridge (Thermo Scientific). The iodoTMT-labelled peptides were enriched by anti-TMT resin as instructed by the manufacturer (Thermo Scientific). Then, the enriched peptides were loaded into liquid chromatography–mass spectrometry for analysis. The Q Exactive HF-X mass spectrometer coupled to Easy nLC1200 (Thermo Scientific) were performed on a 2-h time gradient for peptide mass spectrometry detection. The mass spectrometry raw data were imported into MaxQuant software v220.127.116.11 for data interpretation and protein identification against the B. rapa genome database49 (http://brassicadb.cn). The search results were filtered and exported with a less than 1% false discovery rate at the site level, peptide-spectrum-matched level and protein level. MaxQuant analysis was filtered only for those nitrosated sites (iodoTMT labelled) that were confidently localized (class I, localization probability of more than 0.75) and the score of the modified peptide was more than 40.
All sequences analysed were retrieved from the B. rapa genome database49 (http://brassicadb.cn), NCBI GenBank50 (https://www.ncbi.nlm.nih.gov/genbank) or EnsemblPlants51 (http://plants.ensembl.org/index.html) or Phytozome52 (https://phytozome-next.jgi.doe.gov). C. danica was selected as the outgroup to infer the species relationships among B. rapa, B. oleracea, B. vulgaris, A. thaliana, Raphanus sativus, Arabidopsis lyrata and A. halleri. The maximum likelihood tree based on the alignment of nuclear ribosomal internal transcribed spacers from the above-mentioned species was inferred under a Tamura–Nei nucleotide substitution model with 1,000 bootstraps. For the phylogenetic tree of RBOHs, PCPs and SRKs, corresponding sequences were aligned using the MUSCLE algorithm implemented in MEGA X53, and constructed using the neighbour-joining method in MEGA X with 1,000 bootstraps. The amino acid sequence of PCP-Bs and FER alignment was performed by the online software Clustal Omega54 (https://www.ebi.ac.uk/Tools/msa/clustalo) with default parameters then import into ESPript 3.0 (ref. 55) (https://espript.ibcp.fr/ESPript/cgi-bin/ESPript.cgi) to generate pictures.
Average ROS and NO signals, pollen grain width and pollen tube length were measured by Image J v1.53c (https://imagej.net). Histograms were prepared by GraphPad Prism v8.0.1 (https://www.graphpad-prism.cn). The main figures were assembled in Adobe Illustrator; all other figures were assembled in Adobe Photoshop. Some cartoon components were from www.figdraw.com for model drawing.
Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article.
The source data for Figs. 1–5 and Extended Data Figs. 1–10 are provided with the paper. Raw, uncropped gels and gene accession numbers used in this paper are shown in the Supplementary Information. Source data are provided with this paper.
Broz, A. K. & Bedinger, P. A. Pollen–pistil interactions as reproductive barriers. Annu. Rev. Plant Biol. 72, 615–639 (2021).
Jany, E., Nelles, H. & Goring, D. R. The molecular and cellular regulation of Brassicaceae self-incompatibility and self-pollen rejection. Int. Rev. Cell Mol. Biol. 343, 1–35 (2019).
Nasrallah, J. B. Self-incompatibility in the Brassicaceae: regulation and mechanism of self-recognition. Curr. Top. Dev. Biol. 131, 435–452 (2019).
Lewis, D. & Crowe, L. K. Unilateral incompatibility in flowering plants. Heredity 12, 233–256 (1958).
Kitashiba, H. & Nasrallah, J. B. Self-incompatibility in Brassicaceae crops: lessons for interspecific incompatibility. Breed Sci. 64, 23–37 (2014).
Bedinger, P. A., Broz, A. K., Tovar-Mendez, A. & McClure, B. Pollen–pistil interactions and their role in mate selection. Plant Physiol. 173, 79–90 (2017).
Escobar-Restrepo, J. M. et al. The FERONIA receptor-like kinase mediates male–female interactions during pollen tube reception. Science 317, 656–660 (2007).
Duan, Q. et al. Reactive oxygen species mediate pollen tube rupture to release sperm for fertilization in Arabidopsis. Nat. Commun. 5, 3129 (2014).
Duan, Q. et al. FERONIA controls pectin- and nitric oxide-mediated male–female interaction. Nature 579, 561–566 (2020).
Zhang, L. et al. FERONIA receptor kinase-regulated reactive oxygen species mediate self-incompatibility in Brassica rapa. Curr. Biol. 31, 3004–3016 (2021).
Franklin-Tong, N. & Bosch, M. Plant biology: stigmatic ROS decide whether pollen is accepted or rejected. Curr. Biol. 31, R904–R906 (2021).
Wang, L. et al. PCP-B class pollen coat proteins are key regulators of the hydration checkpoint in Arabidopsis thaliana pollen–stigma interactions. New Phytol. 213, 764–777 (2017).
Doughty, J., Wong, H. Y. & Dickinson, H. G. Cysteine‐rich pollen coat proteins (PCPs) and their interactions with stigmatic S (incompatibility) and S‐related proteins in Brassica: putative roles in SI and pollination. Ann. Bot. 85, 161–169 (2000).
Liu, C. et al. Pollen PCP-B peptides unlock a stigma peptide-receptor kinase gating mechanism for pollination. Science 372, 171–175 (2021).
Murase, K. et al. A membrane-anchored protein kinase involved in Brassica self-incompatibility signaling. Science 303, 1516–1519 (2004).
Gu, T., Mazzurco, M., Sulaman, W., Matias, D. D. & Goring, D. R. Binding of an arm repeat protein to the kinase domain of the S-locus receptor kinase. Proc. Natl Acad. Sci. USA 95, 382–387 (1998).
Kitashiba, H., Liu, P., Nishio, T., Nasrallah, J. B. & Nasrallah, M. E. Functional test of Brassica self-incompatibility modifiers in Arabidopsis thaliana. Proc. Natl Acad. Sci. USA 108, 18173–18178 (2011).
Stone, S. L., Anderson, E. M., Mullen, R. T. & Goring, D. R. ARC1 is an E3 ubiquitin ligase and promotes the ubiquitination of proteins during the rejection of self-incompatible Brassica pollen. Plant Cell 15, 885–898 (2003).
Bailey, C. D. et al. Toward a global phylogeny of the Brassicaceae. Mol. Biol. Evol. 23, 2142–2160 (2006).
Cai, X. et al. Impacts of allopolyploidization and structural variation on intraspecific diversification in Brassica rapa. Genome Biol. 22, 166 (2021).
Nielsen, J. K., Nagao, T., Okabe, H. & Shinoda, T. Resistance in the plant, Barbarea vulgaris, and counter-adaptations in flea beetles mediated by saponins. J. Chem. Ecol. 36, 277–285 (2010).
Badenes-Perez, F. R., Reichelt, M., Gershenzon, J. & Heckel, D. G. Using plant chemistry and insect preference to study the potential of Barbarea (Brassicaceae) as a dead-end trap crop for diamondback moth (Lepidoptera: Plutellidae). Phytochemistry 98, 137–144 (2014).
Nasrallah, M. E., Liu, P. & Nasrallah, J. B. Generation of self-incompatible Arabidopsis thaliana by transfer of two S locus genes from A. lyrata. Science 297, 247–249 (2002).
Luo, X. & Lehotay, D. C. Determination of hydroxyl radicals using salicylate as a trapping agent by gas chromatography–mass spectrometry. Clin. Biochem. 30, 41–46 (1997).
Liszkay, A., van der Zalm, E. & Schopfer, P. Production of reactive oxygen intermediates (superoxide, H2O2, and hydroxyl radical) by maize roots and their role in wall loosening and elongation growth. Plant Physiol. 136, 3114–3123 (2004).
Bechsgaard, J. S., Castric, V., Charlesworth, D., Vekemans, X. & Schierup, M. H. The transition to self-compatibility in Arabidopsis thaliana and evolution within S-haplotypes over 10 Myr. Mol. Biol. Evol. 23, 1741–1750 (2006).
Tsuchimatsu, T. et al. Evolution of self-compatibility in Arabidopsis by a mutation in the male specificity gene. Nature 464, 1342–1346 (2010).
Zhang, T. et al. Generation of transgenic self-incompatible Arabidopsis thaliana shows a genus-specific preference for self-incompatibility genes. Plants (Basel) 8, 570 (2019).
Duan, Q., Kita, D., Li, C., Cheung, A. Y. & Wu, H. M. FERONIA receptor-like kinase regulates RHO GTPase signaling of root hair development. Proc. Natl Acad. Sci. USA 107, 17821–17826 (2010).
Besson-Bard, A., Pugin, A. & Wendehenne, D. New insights into nitric oxide signaling in plants. Annu. Rev. Plant Biol. 59, 21–39 (2008).
Domingos, P., Prado, A. M., Wong, A., Gehring, C. & Feijo, J. A. Nitric oxide: a multitasked signaling gas in plants. Mol. Plant 8, 506–520 (2015).
Guo, F., Okamoto, M. & Crawford, N. M. Identification of a plant nitric oxide synthase gene involved in hormonal signaling. Science 302, 100–103 (2003).
Moreau, M., Lee, G. I., Wang, Y., Crane, B. R. & Klessig, D. F. AtNOS/AtNOA1 is a functional Arabidopsis thaliana cGTPase and not a nitric-oxide synthase. J. Biol. Chem. 283, 32957–32967 (2008).
Tewari, R. K., Prommer, J. & Watanabe, M. Endogenous nitric oxide generation in protoplast chloroplasts. Plant Cell Rep. 32, 31–44 (2013).
Lee, U., Wie, C., Fernandez, B. O., Feelisch, M. & Vierling, E. Modulation of nitrosative stress by S-nitrosoglutathione reductase is critical for thermotolerance and plant growth in Arabidopsis. Plant Cell 20, 786–802 (2008).
Yun, B. W. et al. S-nitrosylation of NADPH oxidase regulates cell death in plant immunity. Nature 13, 264–268 (2011).
Hess, D. T. & Stamler, J. S. Regulation by S-nitrosylation of protein post-translational modification. J. Biol. Chem. 10, 4411–4418 (2012).
Qu, Z. et al. Proteomic quantification and site-mapping of S-nitrosylated proteins using isobaric iodoTMT reagents. J. Proteome Res. 13, 3200–3211 (2014).
Feng, J., Chen, L. & Zuo, J. Protein S-nitrosylation in plants: current progresses and challenges. J. Integr. Plant Biol. 61, 1206–1223 (2019).
Zhan, N. et al. S-nitrosylation targets GSNO reductase for selective autophagy during hypoxia responses in plants. Mol. Cell 71, 142–154 (2018).
Nishi, S., Toda, M. & Toyoda, T. Studies on the embryos culture in vegetable crops. III. On the conditions affecting to embryo culture of interspecific hybrids between cabbage and Chinese cabbage. Bull. Hort. Res. Sta. 9, 75–100 (1970).
Rotman, N. et al. Female control of male gamete delivery during fertilization in Arabidopsis thaliana. Curr. Biol. 4, 432–436 (2003).
Clough, S. J. & Bent, A. F. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 16, 735–743 (1998).
Huang, J. et al. Programmed cell death in stigmatic papilla cells is associated with senescence-induced self-incompatibility breakdown in Chinese cabbage and radish. Front. Plant Sci. 11, 586901 (2020).
Liu, C. & Meinke, D. W. The titan mutants of Arabidopsis are disrupted in mitosis and cell cycle control during seed development. Plant J. 16, 21–31 (1998).
Smyth, D. R., Bowman, J. L. & Meyerowitz, E. M. Early flower development in Arabidopsis. Plant Cell 2, 755–767 (1990).
Jambunathan, N. Determination and detection of reactive oxygen species (ROS), lipid peroxidation, and electrolyte leakage in plants. Methods Mol. Biol. 639, 292–298 (2010).
Li, C. et al. Glycosylphosphatidylinositol-anchored proteins as chaperones and co-receptors for FERONIA receptor kinase signaling in Arabidopsis. eLife 4, e06587 (2015).
Chen, H. X. et al. BRAD V3.0: an upgraded Brassicaceae database. Nucleic Acids Res. 50, 1432–1441 (2022).
Benson, D. A. et al. GenBank. Nucleic Acids Res. 41, 36–42 (2013).
Howe, K. L. et al. Ensembl Genomes 2020-enabling non-vertebrate genomic research. Nucleic Acids Res. 48, 689–695 (2020).
Goodstein, D. M. et al. Phytozome: a comparative platform for green plant genomics. Nucleic Acids Res. 40, 1178–1186 (2012).
Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol. Biol. Evol. 35, 1547–1549 (2018).
Madeira, F. et al. The EMBL-EBI search and sequence analysis tools APIs in 2019. Nucleic Acids Res. 47, 636–641 (2019).
Robert, X. & Gouet, P. Deciphering key features in protein structures with the new ENDscript server. Nucleic Acids Res. 42, W320–W324 (2014).
We thank many people from Shandong Agricultural University for their help in all directions: Y. Hao, Z. Bao and X. Zhang for sharing of equipment, and Z. Ren and C. Zhou for manuscript discussion; and L. Guo from Peking University Institute of Advanced Agricultural Sciences for help in retrieving the genomic sequence of SRK from FPsc. This work was supported by grants from the National Natural Science Foundation of China (32170365 to Q.D.), Shandong Natural Science Foundation (ZR2021ZD07 and ZR2020KC017 to Q.D.), Shandong Yiyi Agricultural Science and Technology Co., Ltd (Q.D.), National Major Scientific Research Program of China (2013CB945100 to Xiansheng Zhang), US National Science Foundation (MCB-1715764 and IOS-2101467 to A.Y.C. and H.-M.W.), and UMass NIFA/USDA (MAS00525 to A.Y.C.).
The authors declare no competing interests.
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Extended data figures and tables
Extended Data Fig. 1 ROS underlies the rejection of interspecific pollen during UI.
a, The developmentally regulated SI and the progressive increase in the amount of SRK from bud stage to maturity. b, The number of intra– and various interspecific pollen tubes in mature or bud-stage B. rapa stigmas. Mature B. rapa stigmas rejected SI and all the interspecific pollen examined, but bud-stage B. rapa stigmas allowed the growth of SI pollen and interspecific B. oleracea pollen, and still rejected intergeneric B. vulgaris and A. thaliana pollen. See Fig. 1b. c, Imaging of H2DCFDA-stained ROS under confocal microscope and under wide-field fluorescence microscope showed comparable changes of ROS in B. rapa stigmas Dotted line outlined the area for ROS quantification. Wide-field observation, with its considerably high expedition to sample entire stigma specimens, was used for the massive amount of data gathering required for this study. d, ROS intensity in unpollinated (UP) or pollinated B. rapa stigma. See Fig. 1c. e–g, Scavenging ROS by Na-SA suppressed stigmatic ROS induction, and promoted the growth of SI pollen and B. oleracea pollen in mature and B. vulgaris pollen in bud-stage B. rapa stigmas. Scale bars, 500 μm. Data bar (a): average ± SD. Average relative expression levels from three biological replicates of stigmas (two tailed t-test, n = 3). Box plots (b–g): centre line, median; box limits, lower and upper quartiles; dots, individual data points; whiskers, highest and lowest data points. n (in blue), number of stigmas. P values, two-tailed t-tests. Each experiment was repeated at least thrice with consistent results.
Extended Data Fig. 2 SRK underlies the rejection of interspecific pollen during UI.
a, Quantitative RT-PCR showing AS-BrSRK46 treatment reduced BrSRK46 expression in mature B. rapa stigmas (S46) and further reduced its already low level in bud-stage stigmas (S46). b, AS-BrSRK46 treatment of mature B. rapa stigmas (S46) broke the inhibition to B. oleracea pollen not B. vulgaris pollen. AS-BrSRK46 treatment of bud-stage stigmas (S46) broke inhibition to B. vulgaris pollen. See Fig. 1d. c, d, AS-BrSRK46 treatment reduced ROS in mature B. rapa stigmas (S46) and further reduced its already low ROS in bud-stage stigmas (S46). e–g, Sequence alignment (e), phylogenetic analysis (f) and expression of SRK in FPsc (Brara.G02663) (g). SRK from FPsc lacks the coding sequence for the transmembrane domain and we name it BrSRK∆TM hereafter. h–j, Mature and bud-stage stigmas of FPsc were defective in SI and UI pollen-triggered ROS increase (h, i) and the rejection of SI and UI pollen (j). See Fig. 1e. k, SRK13 expression from SI A. thaliana stigmas transformed with Arabidopsis halleri AhSP11/SCR13-AhSRK13-AhARC1. These three transgenes were in a single construct. l, m, The number of intra– or interspecific pollen tubes (l) and ROS changes (m) in SC A. thaliana stigmas and in SI A. thaliana stigmas. See Fig. 1f, g. n, B. oleracea pollen of S36-haplotype was rejected in B. rapa stigmas of S46, S12, S9, S40, or S38, showing the dependence on SRK but the independence of SCR-SRK interaction in the rejection of interspecific pollen. Scale bars, 500 μm (c, d, h, i, n). Data bars (a, g, k): average ± SD. Average relative expression levels from three biological replicates of stigmas (two tailed t-test, n = 3). Box plots (b–d, h–j, l–n): centre line, median; box limits, lower and upper quartiles; dots, individual data points; whiskers, highest and lowest data points. n (in blue), number of stigmas. P values, two-tailed t-tests. Each experiment was repeated at least thrice with consistent results.
Extended Data Fig. 3 FER/ANJ signalling regulates stigmatic ROS during UI.
a, b, Quantitative RT-PCR showing AS-BrFER1 treatment suppressed the expression of BrFER1(a) and promoted the growth of SI pollen and B. oleracea pollen in mature B. rapa stigmas and B. vulgaris pollen in bud stage (b) B. rapa stigmas. See Fig. 2a. c, d, AS-BrFER1 treatment suppressed SI and UI-induced ROS increase in mature and bud stage B. rapa stigmas at 10 MAP. e, f, The expression of FER and SRK in stigmas of A. tha (SI)/fer-4 (e) and loss of FER in SI A. thaliana promoted the growth of SI pollen, B. oleracea pollen and B. vulgaris pollen in SI A. thaliana stigmas (f). See Fig. 2b. g, Loss of FER in SI A. thaliana suppressed SI and UI-induced ROS increase in A. tha (SI)/fer-4 stigmas at 10 MAP. h, Domain structures of BrANJs proteins showing only BrANJ1 have intact extracellular and intracellular domain. i, j, AS-BrANJ1 treatment suppressed the expression of BrANJ1(i) and promoted the growth of SI pollen and B. oleracea pollen in mature B. rapa stigmas and B. vulgaris pollen in bud stage B. rapa stigmas (j). k, l, AS-BrANJ1 treatment suppressed SI and UI-induced ROS increase in mature (k) and bud stage (l) B. rapa stigmas at 10 MAP. Scale bars, Scale bars, 500 μm (c, d, j, k, l), 200 μm (g). Data bars (a, e, i): average ± SD. Average relative expression levels from three biological replicates of stigmas (two tailed t-test, n = 3). Box plots (b–d, f, g, j–l): centre line, median; box limits, lower and upper quartiles; dots, individual data points; whiskers, highest and lowest data points. n (in blue), number of stigmas. P values, two-tailed t-tests. Each experiment was repeated at least thrice with consistent results.
Extended Data Fig. 4 ROS are produced by RBOHs during UI.
a, Quantitative RT-PCR showing AS-BrRBOHF treatment suppressed the expression of BrRBOHF. b, c, AS-BrRBOHF treatment suppressed SI and UI-induced ROS increase in mature (b) and bud-stage (c) B. rapa stigmas at 10 MAP. d, The RBOH enzymatic activity in mature B. rapa stigmas were increased after pollination with SI and UI pollen but decreased after pollination with CP pollen. e, AS-BrRBOHF treatment promoted the growth of SI pollen and B. oleracea pollen in mature and B. vulgaris pollen in bud-stage B. rapa stigmas. Scale bars, 500 μm. Data bar (a): average ± SD. Average relative expression levels from three biological replicates of stigmas (two tailed t-test, n = 3). Data bars (d): Average activity of ROS producing enzymes from three technical replicates of one stigma sample containing 100 stigmas (two tailed t-test, n = 3). Box plots (b, c, e): centre line, median; box limits, lower and upper quartiles; dots, individual data points; whiskers, highest and lowest data points. n (in blue), number of stigmas. P values, two-tailed t-tests. Each experiment was repeated at least thrice with consistent results unless otherwise specified.
Extended Data Fig. 5 SRK is correlated with stigmatic ROS during UI.
a, b, BrSRK46 kinase domain (KD) interacts with BrFER1 (KD) in yeast two-hybrid assay (a) and BiFC (b). c, d, Protein extract from SI pollen and B. oleracea pollen (S36), but not that from CP pollen (S12), increased ROS in B. rapa stigmas (S46) (c), and enhanced interaction between BrSRK46 and BrFER1 (d) in the pull-down assay. See Fig. 2c. e–g. ROS in B. rapa stigmas (S46) were increased (e), and the BrSRK46-BrFER1 interaction enhanced in the pull-down (f) and Co-IP assays (g), by GST-BrSCR46, not GST-BrSCR12, which is active for S12 stigma. The protein samples were derived from the same experiment and the blots were processed in parallel (d, f, g). For gel source data, see Supplementary Fig. 1. See Fig. 2d, e. h, i, Nitro blue tetrazolium staining or tobacco leaves and H2DCFDA staining of protoplasts from tobacco leaves co-expressing BrFER1, BrRBOHD2, and BrSRK46 showing the enhancement of ROS by BrSCR46, not by BrSCR12. Scale bars, 500 μm (c, e); 50 μm (i). Data bars (d, f, g): average ± SD. Average relative intensities from three biological replicates of the blots shown in Fig. 2c–e (two tailed t-test, n = 3). Box plots (c, e, h, i): centre line, median; box limits, lower and upper quartiles; dots, individual data points; whiskers, highest and lowest data points. n (in blue), number of stigmas, tobacco leaves, protoplast samples. P values, two-tailed t-tests. Each experiment was repeated at least thrice with consistent results.
Extended Data Fig. 6 The relationship of FER-ROS signaling with MLPK and ARC1 during SI and UI.
a, b, Quantitative RT-PCR showing AS-BrMLPK and AS-BrARC1 treatment suppressed the expression of BrMLPK and BrARC1, respectively, in mature B. rapa stigmas. c, d, AS-BrMLPK (c) and AS-BrARC1 (d) treatment promoted the growth of SI and UI pollen tubes in mature B. rapa stigmas (S46). e, AS-BrMLPK treatment of mature B. rapa stigmas (S46) inhibited SI and UI pollen-induced ROS increase. f, AS-BrARC1 treatment of mature B. rapa stigmas (S46) did not affect SI and UI pollen-induced ROS increase. Scale bars, 500 μm. Data bars (a, b): average ± SD. Average relative expression levels from three biological replicates of stigmas (two tailed t-test, n = 3). Data bar: average ± s.d. Box plots (c–f): centre line, median; box limits, lower and upper quartiles; dots, individual data points; whiskers, highest and lowest data points. n (in blue), number of stigmas. P values, two-tailed t-tests. Each experiment was repeated at least thrice with consistent results.
Extended Data Fig. 7 Species-specific match of PCP-Bs and FER.
a–c, When observed at 1.5 HAP, the reduced reproductive barrier to B. rapa and B. oleracea pollen in A. thaliana fer mutant (a, srn) and rbohd mutant (b, c) stigmas was obvious. See also Fig. 3c. d, Phylogenetic analysis of PCP-B genes from A. thaliana, B. oleracea, and B. rapa. We name BrapaB312, an ortholog of AtPCP-Bγ, BrPCP-B3 hereafter. e, Amino acid sequence alignment of PCP-Bs from A. thaliana, B. oleracea, and B. rapa shows the comparison of PCP-B sequences. They all shared a common pattern of seven or eight cysteines in the mature polypeptide. Red rectangle shows the conserved cysteines (C30, C32, C33 of AtPCP-B), mutation of which render a loss in the ability for stigmatic ROS reduction and pollen hydration14. f, Synthetic AtPCP-Bγ and recombinant MBP-AtPCP-Bγ show similar function in reducing ROS of UP A. thaliana stigmas, but GST-BrPCP-B3 shows no effect in reducing ROS at 5 or 10 MAT. g, B. rapa stigmas and A. thaliana stigmas, which express divergent FER, responded species-preferentially to BrPCP-B3 in reducing ROS. h, AtPCP-Bγ was much faster than GST-BrPCP-B3 in reducing ROS of A. thaliana roots, which also express FER28. i, GST-BrPCP-B3 was more effective in reducing ROS of B. rapa roots than that of A. thaliana roots. j, Quantified data of pull-down assay showing the inefficiency of GST-BrPCP-B3 to compete with AtPCP-Bγ in interaction with AtFER (ED)-FLAG. The protein samples were derived from the same experiment and the blots were processed in parallel. For gel source data, see Supplementary Fig.1. See also Fig. 3f. Scale bars, 200 μm (a–c, f, g) and roots (h, i). Data bar (j): average ± SD. Average relative intensities from three biological replicates of the blot represented on the left (two tailed t-test, n = 3). Box plots (a–c, f, g–i): centre line, median; box limits, lower and upper quartiles; dots, individual data points; whiskers, highest and lowest data points. n (in blue), number of stigmas or roots. P values, two-tailed t-tests. Each experiment was repeated at least thrice with consistent results.
Extended Data Fig. 8 Stigmatic NO is required for ROS reduction in A. thaliana stigmas during SC and UC.
a, b, Confocal and wide-field imaging showing the increase of stigmatic NO at 5 MAP with SC pollen (a) and quantified data of SC-induced NO and pollen hydration in A. thaliana stigmas, both showed NO peaking at ~5 MAP (b). See Fig. 4a. c, d, FER-dependent elevation of NO in A. thaliana stigmas induced by pollen and At-PCP-Bγ .See Fig. 4d, e. e, AtPCP-Bγ was much faster than GST-BrPCP-B3 in increasing NO of A. thaliana roots. f, GST-BrPCP-B3 was more effective in increasing NO of B. rapa roots than that of A. thaliana roots. g, Roots of fer-4 was not responsive to AtPCP-Bγ in inducing NO. h, i, (Left to right) cPTIO scavenged SC-induced NO, suppressed SC-induced ROS reduction, and inhibited SC pollen hydration and tube growth in A. thaliana stigmas. j–n, Relative to WT, noa1 stigmas showed lower NO and higher ROS levels, and slower hydration of SC (WT A. thaliana) pollen and pollen tube growth (j, k, l). Relative to WT, hot5-4 stigmas showed faster NO and lower ROS levels, and faster hydration of SC (WT A. thaliana) pollen and pollen tube growth (j, m, n). o, p, Mutations in NOA1, noa1 (o) and GSNOR, hot5-4 (p), enhanced and reduced, respectively, the reproductive barrier to B. rapa or B. oleracea pollen in A. thaliana stigmas at 1.5 HAP. Scale bars, 200 μm (a, h, j, o, p) and roots (e–g); 50 μm for pollen (h, j). Box plots (a–p): centre line, median; box limits, lower and upper quartiles; dots, individual data points; whiskers, highest and lowest data points. n (in blue), number of stigmas, pollen grains or roots. P values, two-tailed t-tests. Each experiment was repeated at least thrice with consistent results.
Extended Data Fig. 9 The inverse relationship of NO and ROS and nitrosation of BrFER1 and RBOHs in B. rapa stigmas.
a, b, Intraspecific CP pollen (2nd row), but not SI and B. oleracea (UI) pollen, induced NO in B. rapa stigmas. c–e, Scavenging NO increased ROS (c) and RBOH enzymatic activity of UP B.rapa stigmas (d), and inhibited the growth of CP pollen tubes in B. rapa stigmas (e). f–h, GSNO treatment increased NO (f), reduced ROS (f) and RBOH enzymatic activity of UP B. rapa stigmas (g), and promoted the growth of SI or B. oleracea pollen in mature or B. vulgaris polen in bud-stage B. rapa stigmas (h). i, BrFER1(KD), not BrFER1 (ED), was nitrosated by GSNO. See also Fig. 4f. j, k, LC-MS spectrum showing the nitrosated cysteine residues in BrFER1 protein. l, FER amino acid sequence alignment showing the nitrosated cysteines, Cys730 and Cys752 (*) are evolutionarily conserved. m, Pull-down assay shows GSNO treatment induced quantitative inhibition of MBP-BrFER1(KD) with GST-BrROP2 complex. See also Fig. 4h. n, The nitrosomimetic mutation, Cys730W, reduced the amount of BrFER1 that was pulled down by GFP-BrROP2. o, Phylogenetic analysis of BrRBOHs and AtRBOHD. BrRBOHD1 and BrRBOHD2 are closer to AtRBOHD than other BrRBOHs. p–r, RBOHD was nitrosated by NO. In vitro nitrosation of BrRBOHD1 by GSNO (p); CP-induced nitrosation of GFP-AtRBOHD protein from A. thaliana stigmas (q), and LC–MS spectra showing nitrosation of Cys891 of GST-BrRBOHD1 (CT, C-terminal) (r). Nitrosated amino acid residues were labelled with TMT. Proteins were representative of more than three independent preparations. s, t, GST-BrRBOHD2 (CT) (s) and GST-BrRBOHF (CT) (t) proteins were nitrosated in vitro. The protein samples were derived from the same experiment and the blots were processed in parallel (i, m, n, p, q, s, t). For gel source data, see Supplementary Fig. 1. Scale bars, 500 μm. Line chart (b): average ± SD. Average relative NO intensities from three biological replicates of stigmas shown in a (two tailed t-test, n = 3). Data bars (d, g): Average activity of ROS producing enzymes from three technical replicates of one stigma sample containing 100 stigmas (two tailed t-test, n = 3). Data bar (m, n): average ± SD. Average relative intensities from three biological replicates of stigmas in (a) and blots in Fig 4h and in (n) (two tailed t-test, n = 3). Box plots (c, e, f, h): centre line, median; box limits, lower and upper quartiles; dots, individual data points; whiskers, highest and lowest data points. n (in blue), number of stigmas. P values, two-tailed t-tests. Each experiment was repeated at least thrice with consistent results unless otherwise specified.
Extended Data Fig. 10 Breaking interspecific barriers in Brassica crops.
a, b, Reducing ROS by Na-SA or increasing NO by GSNO increased the number of enlarged ovules in the pod of ♀B. rapa × ♂B. oleracea or ♀B. rapa × ♂B. vulgaris. See Fig. 5a. c, Suppressing the expression of BrSRK46, BrBrFER1, or BrRBOHF by AS-ODNs increased the number of enlarged ovules in the pod of ♀B. rapa × ♂B. oleracea or ♀B. rapa × ♂B. vulgaris. See also Fig. 5b. d, B. oleracea pollen tubes or B. vulgaris pollen tubes in B. rapa pistils. The reduced number of enlarged ♀B. rapa × ♂B. vulgaris ovules is possibly due to fewer B. vulgaris pollen tubes had exited the transmitting tissue and targeted the B. rapa ovules due to additional barriers based on their evolutionary distance (Fig. 1a). Arrowheads point to bundles of pollen tubes in the transmitting tissue, stars point to ovules penetrated by a pollen tube. Scale bars, 100 μm (d). Box plots (a–c): centre line, median; box limits, lower and upper quartiles; dots, individual data points; whiskers, highest and lowest data points. n (in blue), number of pistils. P values, two-tailed t-tests. Each experiment was repeated at least thrice with consistent results.
This file contains Supplementary Fig. 1 and Supplementary Tables 1–4.
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Huang, J., Yang, L., Yang, L. et al. Stigma receptors control intraspecies and interspecies barriers in Brassicaceae. Nature 614, 303–308 (2023). https://doi.org/10.1038/s41586-022-05640-x
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