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Antiviral signaling by a cyclic nucleotide activated CRISPR protease

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Abstract

CRISPR defense systems such as the well-known DNA-targeting Cas9 and the RNA-targeting type III systems are widespread in prokaryotes1,2. The latter can orchestrate a complex antiviral response that is initiated by the synthesis of cyclic oligoadenylates (cOAs) upon foreign RNA recognition3–5. Among a large set of proteins that were linked to type III systems and predicted to bind cOAs6,7, a CRISPR associated Lon protease (CalpL) stood out to us. The protein contains a sensor domain of the SAVED (SMODS-associated and fused to various effector domains) family7, fused to a Lon protease effector domain. However, the mode of action of this effector was unknown. Here, we report the structure and function of CalpL and show that the soluble protein forms a stable tripartite complex with two further proteins, CalpT and CalpS, that are encoded in the same operon. Upon activation by cA4, CalpL oligomerizes and specifically cleaves the MazF-homolog CalpT, releasing the extracytoplasmic function (ECF) sigma factor CalpS from the complex. This provides a direct connection between CRISPR-based foreign nucleic acid detection and transcriptional regulation. Furthermore, the presence of a cA4-binding SAVED domain in a CRISPR effector reveals an unexpected link to the cyclic oligonucleotide-based antiphage signaling system (CBASS).

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Correspondence to Christophe Rouillon or Gregor Hagelueken.

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This file contains Supplementary Fig. 1, showing the uncropped and unedited versions of all gels used in this study.

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Rouillon, C., Schneberger, N., Chi, H. et al. Antiviral signaling by a cyclic nucleotide activated CRISPR protease. Nature (2022). https://doi.org/10.1038/s41586-022-05571-7

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  • DOI: https://doi.org/10.1038/s41586-022-05571-7

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