Extended Data Fig. 2: Erythroid differentiation of edited SCD CD34+ HSPCs. | Nature

Extended Data Fig. 2: Erythroid differentiation of edited SCD CD34+ HSPCs.

From: Base editing of haematopoietic stem cells rescues sickle cell disease in mice

Extended Data Fig. 2

Representative, immuno-flow cytometry for erythroid maturation stage markers42,43 at culture days 7 and 14. Top, gating strategy to identify single cells expressing the erythroid marker hCD235a. Bottom, gating strategy to track the progress of erythroid maturation based on expression of CD49D and BAND3 in hCD235a+ cells. SSC-A, side scatter area; SSC-W, side scatter width; FSC-A, forward scatter area.

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