a, viSNE analysis of total CD45+ cells to identify immune cell types. Representative viSNE plots are from a Vav-iCre+/− control mouse at 10–12 months of age. See Supplemental Fig. 5 for the gating strategy. b, The proportion of the indicated immune cell subsets that express p16, p21 or CENP-B from 10–12-month-old Vav-iCre+/−;Ercc1−/fl (n = 6), Vav-iCre+/− (n = 6), and >2-year-old wild-type (n = 7) mice was determined by CyTOF. Each dot is an independent mouse. Data are mean ± s.d. *P < 0.05, **P < 0.01, ***P < 0.001, Kruskal–Wallis test with Dunn’s correction for multiple comparisons.