Extended Data Fig. 3: Measurement of immune function and lymphoid organ senescence in Vav-iCre+/−;Ercc1−/fl mice. | Nature

Extended Data Fig. 3: Measurement of immune function and lymphoid organ senescence in Vav-iCre+/−;Ercc1−/fl mice.

From: An aged immune system drives senescence and ageing of solid organs

Extended Data Fig. 3

a, Footpad swelling measurements at several time points after antigenic (KLH) challenge, separated by mouse genotype (n = 7 Vav-iCre+/−;Ercc1−/fl; n = 6 Vav-iCre+−; n = 5 old WT mice). b, Footpad swelling by genotype (n = 7 Vav-iCre+/−;Ercc1−/fl; n = 6 Vav-iCre+/−; n = 5 WT mice). c, Quantification of anti-KLH antibodies by ELISA one month after antigenic challenge (n = 3/7 Vav-iCre+/−;Ercc1−/fl; n = 3/6 Vav-iCre+/−; n = 3/3 Ercc1−/fl for naive (uninjected) and KLH-challenged (injected) mice, respectively). d, DTH assay in 2-month-old mice Vav-iCre+/−;Ercc1−/fl (n = 5) and Vav-iCre+/ (n = 8) controls. Data are mean ± s.d. ∞P < 0.01, P < 0.001, #P < 0.0001, two-way ANOVA with Tukey’s test. e, Quantification of Ercc1 expression by qRT–PCR in flow-sorted immune cell populations isolated from spleen (T and natural killer cells) and bone marrow (B cells and macrophages) of 5-month-old Vav-iCre+/−;Ercc1−/fl mice and littermate controls (n = 4 Vav-iCre+/−;Ercc1−/fl; n = 3 Vav-iCre+/). Data are mean ± s.d. P < 0.001, #P < 0.0001, two-tailed unpaired Student’s t-test. f, Senescence marker expression in CD3+ peripheral T cells from 8–11-month-old Vav-iCre and old wild-type mice (n = 3–9 Vav-iCre+/−; n = 6–9; Vav-iCre+/−;Ercc1−/fl; n = 4–7 two-year-old wild-type, depending on the gene) (see Supplementary Table 3 for sample size details). g, Measurement of senescence marker expression by qRT–PCR in splenic tissue and bone marrow from 2–3-month-old Vav-iCre mice (n = 3). h, Expression of senescence markers in splenic tissue from 8–10-month-old Vav-iCre+/−;Ercc1−/fl and old wild-type mice relative to controls by gender (n = 3–4/3–4 Vav-iCre+/; n = 3–5/3–4 Vav-iCre+/−;Ercc1−/fl; n = 3–6/3–5 4-month-old WT; n = 5/5 two-year-old WT males/females, respectively) (see Supplementary Table 3 for sample size details). i, Expression of senescence markers in bone marrow of Vav-iCre+/−;Ercc1−/fl mice (n = 3–4/3–4 Vav-iCre+/−; n = 3–4/4–5 Vav-iCre+/−;Ercc1−/fl males/females, respectively). Data are mean ± s.d. *P < 0.05, ∞P < 0.01, P < 0.001, #P < 0.0001, unpaired two-tailed Student’s t-test (e, g), one-way ANOVA (f) or two-way ANOVA (ad, h, i) with Tukey’s test.

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