Extended Data Fig. 4: All extruded cells display features of cell cycle entry, S-phase arrest, and replication stress. | Nature

Extended Data Fig. 4: All extruded cells display features of cell cycle entry, S-phase arrest, and replication stress.

From: Replication stress promotes cell elimination by extrusion

Extended Data Fig. 4

ad, tDHB–GFP fluorescence in unidentified extruded cells from the anterior sensory depression (b), the ventral pocket (c), and the posterior tip (d) of a comma stage embryo of the genotype heSi192; ced-3(lf); nIs861 after RNAi against empty vector control. Nuclei of extruded cells, as identified by Nomarski optics, are marked by dotted outlines. e, f, Micrographs of GFP–PCN-1 fluorescence in unidentified extruded cells (arrowhead) at the ventral pocket (e) or the anterior sensory depression (f) from ced-3(lf); isIs17; nIs861 embryos after RNAi against empty vector control (e) or no RNAi (f). Insets, extruded cells marked by arrowheads in micrographs. gj, RPA-1–YFP fluorescence in unidentified extruded cells from the anterior sensory depression (h, i) and ventral pocket (j) in a ced-3(lf); ltIs44; opIs263[Prpa-1::rpa-1::YFP] embryo after RNAi against empty vector control. Scale bars, 10 μm.

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