Extended Data Fig. 3: ABplpappap, which is generated by an unequal cell division, arrests in S phase and is extruded. | Nature

Extended Data Fig. 3: ABplpappap, which is generated by an unequal cell division, arrests in S phase and is extruded.

From: Replication stress promotes cell elimination by extrusion

Extended Data Fig. 3

a, b, Confocal fluorescence micrographs of tDHB–GFP fluorescence in ABplpappap (arrowheads) before (a) and after (b) ventral enclosure in heSi192[Peft-3::tDHB-GFP]; ced-3(lf); nIs861[Pegl-1::mCherry::PH] embryos after the indicated RNAi treatment. Dotted outline, ABplpappap nucleus, as identified by Nomarski optics. c, d, Confocal fluorescence micrographs of GFP–PCN-1 fluorescence in ABplpappap (arrowheads) before (c) and after (d) ventral enclosure in ced-3(lf); isIs17[Ppie-1::GFP::pcn-1]; nIs861 embryos after the indicated RNAi treatment. e, Time-lapse confocal fluorescence micrographs of GFP–PCN-1 fluorescence in ABplpappap (arrowheads) in a ced-3(lf); isIs17; nIs861; pig-1(RNAi) embryo at the indicated times. tve, time point of ventral enclosure. fh, Micrographs of virtual lateral section of ced-3(lf); nIs861; stIs10026 embryos showing either ABplpappap (arrowhead) or its daughter cells (arrowheads) after indicated RNAi treatment. im, Confocal fluorescence micrographs of ced-3(lf); ltIs44[Ppie-1::mCherry::PH]; stIs10026 embryos showing the relative sizes of ABplpappap and its sister cell, ABplpappaa, in embryos after the indicated RNAi treatment. Insets, ABplpappap (ad); ABplpappap or its daughters (e–h); magnified view of the region indicated, which includes ABplpappap (†) and ABplpappaa (*) (im). Scale bars, 10 μm.

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