Extended Data Fig. 1: Controls for two-choice odour-preference assay. | Nature

Extended Data Fig. 1: Controls for two-choice odour-preference assay.

From: Hunger enhances food-odour attraction through a neuropeptide Y spotlight

Extended Data Fig. 1

a, Timeline of two-choice behavioural assay. b, c, Odour investigation times of fed (b) and fasted (c) male and female wild-type mice in single odour pairings with water. n (for b) = 10 male mice and 10 female mice; n (for c) = 3 male and 3 female mice. Data are mean ± s.e.m., lines with triangles represent individual mice. *P < 0.05, ***P < 0.001, two-tailed Wilcoxon test (P left to right in b: <0.0001, <0.0001, 0.86; P left to right in c: 0.03, 0.03). A statistical comparison of odour responses in b and c using a two-tailed Mann–Whitney U test revealed significant increases in food-odour investigation in fasted mice compared with fed mice (P = 0.0006) but not in pheromone investigation (P = 0.15). d, Odour investigation times of fed (left) and fasted (right) wild-type female mice. n = 10 mice, data are mean ± s.e.m., lines with triangles represent individual mice. **P < 0.01, two-tailed Wilcoxon test (P fed: 0.77, P fasted: 0.002), e, Odour-investigation times of male (left) and female (right) wild-type mice fed ad libitum. n = 10 mice, data are mean ± s.e.m., lines with triangles represent individual mice, P male, 0.92; P female, 0.19; two-tailed Wilcoxon test.

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