Extended Data Fig. 7: Odour preferences and food consumption patterns in knockout and rescue mice. | Nature

Extended Data Fig. 7: Odour preferences and food consumption patterns in knockout and rescue mice.

From: Hunger enhances food-odour attraction through a neuropeptide Y spotlight

Extended Data Fig. 7

a, Timeline of two-choice behavioural assay. b, Investigation times for pheromones and food odours in fed (top) and fasted (bottom) in the indicated female mice. n = 10 mice, data are mean ± s.e.m., lines with triangles represent individual mice. **P < 0.01, two-tailed Wilcoxon test (from left to right, P fed: 0.08, 0.16, 0.24; P fasted: 0.002, 0.002, 0.85). c, Food intake before two-choice assay (Food 1 in timeline) of various knockout male (left) and female (right) mice. n = 10 mice, data are mean ± s.e.m., triangles represent individual mice. d, Cartoon (left) and preference indices (right) of NPY rescue experiments involving Npy-KO (control) and Agrp-ires-cre;Npy-KO (NpyAGRP rescue) mice three weeks after AAV-lsl-Npy injection in the arcuate nucleus. The schematic of the brain is based on published brain section images31. NPY immunohistochemistry is depicted in the figure inset. Preference indices are derived from data in Fig. 3d (n = 12 mice, males and females, mean ± s.e.m. Scale bar, 100 μm). e, Timeline of two-choice behavioural assay for receptor knockouts. f, Investigation times for pheromones and food odours in the fasted female mice indicated. n = 10 mice, data are mean ± s.e.m., lines with triangles represent individual mice. **P < 0.01, two-tailed Wilcoxon test. (P Npy1r-KO: 0.02; P Npy5r-KO: 0.77). g, Food intake after two-choice assay (Food 2 in timeline) of the indicated male and female mice. n = 10 mice, data are mean ± s.e.m., triangles represent individual mice.

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