Fig. 4: 4T-Trap synergizes with VEGF-Trap to induce cancer cell death and suppress tumour growth. | Nature

Fig. 4: 4T-Trap synergizes with VEGF-Trap to induce cancer cell death and suppress tumour growth.

From: Cancer immunotherapy via targeted TGF-β signalling blockade in TH cells

Fig. 4

a, Representative images of HPP (red) and CD31 (white) and VEGFA (green) immunofluorescence in tumour tissues from CD4 PyMT mice treated with mGO53 or 4T-Trap. The percentage of HPP+VEGFAhi areas was calculated from 1-mm2 regions (n = 6 for each group). Scale bars, 100 μm. b, Representative images of HPP (white) and CD31 (red), CC3 (blue) and E-cadherin (green) immunofluorescence in tumour tissues from mice treated with the indicated antibodies. The percentage of CD31+, HPP+ or CC3+ areas as a proportion of E-cadherin+ epithelial regions was calculated from 1-mm2 regions (n = 10 for each group). Isolated CD31+ staining was counted from 1-mm2 regions (n = 10 for each group). Scale bars, 100 μm. c, Representative high-magnification images of HPP (white) and CD31 (red), CC3 (blue) and E-cadherin (green) immunofluorescence in tumour tissues from mice treated with the indicated antibodies. The smallest distance of HPP+ regions (magenta dashed lines) or CC3+ regions (yellow dashed lines) to CD31+ endothelial cells was measured and plotted (n = 7 for each group). Scale bars, 20 μm. d, Tumour measurements from CD4 PyMT mice treated with the indicated antibodies (n = 7 for each group). e, Kaplan–Meier survival curve of CD4 PyMT mice treated with mGO53, 4T-Trap, VEGF-Trap or both 4T-Trap and VEGF-Trap (n = 10, 9, 10 and 8, respectively). Data are mean ± s.e.m.; ***P < 0.001, ****P < 0.0001; NS, not significant; two-tailed unpaired t-test (a) or paired t-test (c), two-way ANOVA with post hoc Bonferroni t-test (b, d) or log-rank test (e). Data are pooled biological replicates (d, e) or representative of three independent experiments (ac).

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