Fig. 3: Blockage of TGF-β signalling in CD4+ T cells induces tumour tissue healing, vessel reorganization and hypoxia-associated cancer cell death. | Nature

Fig. 3: Blockage of TGF-β signalling in CD4+ T cells induces tumour tissue healing, vessel reorganization and hypoxia-associated cancer cell death.

From: TGF-β suppresses type 2 immunity to cancer

Fig. 3

a, Representative immunofluorescence images of fibrinogen (white), CD31 (red), CC3 (cyan) and E-cadherin (green) in individual tumours of around 8 × 8 mm from 23-week-old Tgfbr2fl/fl PyMT (WT) and Thpok-cre;Tgfbr2fl/fl PyMT (KO) mice. Extravascular fibrinogen deposition events (magenta arrows) were calculated from 1-mm2 regions (n = 9 (Tgfbr2fl/fl PyMT) and 9 (Thpok-cre;Tgfbr2fl/fl PyMT)). Isolated CD31 staining (yellow arrows) was counted from 1-mm2 regions (n = 6 (Tgfbr2fl/fl PyMT) and 6 (Thpok-cre;Tgfbr2fl/fl PyMT)). b, Representative immunofluorescence images of NG2 (white), CD31 (red), GP38 (cyan) and E-cadherin (green) in tumours from Tgfbr2fl/fl PyMT and Thpok-cre;Tgfbr2fl/fl PyMT mice. NG2-unbound (magenta arrows) or GP38-unbound (yellow arrows) isolated CD31+ staining was counted from 1-mm2 regions (n = 9 (Tgfbr2fl/fl PyMT) and 9 (Thpok-cre;Tgfbr2fl/fl PyMT)). c, Representative immunofluorescence images of collagen IV (Col IV, white), CD31 (red), fibronectin (cyan) and E-cadherin (green) in tumours from Tgfbr2fl/fl PyMT and Thpok-cre;Tgfbr2fl/fl PyMT mice. The average continuous lengths of collagen IV and fibronectin were measured in 1-mm2 regions (n = 9 (Tgfbr2fl/fl PyMT) and 9 (Thpok-cre;Tgfbr2fl/fl PyMT)). d, Representative immunofluorescence images of a hypoxia probe (HPP, white), CD31 (red), CC3 (cyan) and E-cadherin (green) in tumours from Tgfbr2fl/fl PyMT and Thpok-cre;Tgfbr2fl/fl PyMT mice. The percentage of HPP+E-cadherin+ regions over E-cadherin+ epithelial regions was calculated from 1-mm2 regions (n = 9 (Tgfbr2fl/fl PyMT) and 9 (Thpok-cre;Tgfbr2fl/fl PyMT)). The shortest distance from HPP+ regions (magenta dashed lines) or CC3+ regions (yellow dashed lines) to CD31+ endothelial cells was measured in tumour tissues from Thpok-cre;Tgfbr2fl/fl PyMT mice (n = 9). Data are mean ± s.e.m. (biologically independent mice in ad). Two-tailed unpaired (ac) or paired (d) t-test. The dashed boxes coupled with dashed lines show regions that are further magnified in the bottom panels.

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