Infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is initiated by virus binding to the ACE2 cell-surface receptors1,2,3,4, followed by fusion of the virus and cell membranes to release the virus genome into the cell. Both receptor binding and membrane fusion activities are mediated by the virus spike glycoprotein5,6,7. As with other class-I membrane-fusion proteins, the spike protein is post-translationally cleaved, in this case by furin, into the S1 and S2 components that remain associated after cleavage8,9,10. Fusion activation after receptor binding is proposed to involve the exposure of a second proteolytic site (S2′), cleavage of which is required for the release of the fusion peptide11,12. Here we analyse the binding of ACE2 to the furin-cleaved form of the SARS-CoV-2 spike protein using cryo-electron microscopy. We classify ten different molecular species, including the unbound, closed spike trimer, the fully open ACE2-bound trimer and dissociated monomeric S1 bound to ACE2. The ten structures describe ACE2-binding events that destabilize the spike trimer, progressively opening up, and out, the individual S1 components. The opening process reduces S1 contacts and unshields the trimeric S2 core, priming the protein for fusion activation and dissociation of ACE2-bound S1 monomers. The structures also reveal refolding of an S1 subdomain after ACE2 binding that disrupts interactions with S2, which involves Asp61413,14,15 and leads to the destabilization of the structure of S2 proximal to the secondary (S2′) cleavage site.
Recognition of the ACE2 receptor by the membrane spike glycoprotein of SARS-CoV-2 is a major determinant of virus infectivity, pathogenesis and host range. Previous structural studies on the spike glycoproteins of coronaviruses6,16,17,18,19,20,21,22 have shown that the spike trimer consists of a central helical stalk—comprising three interacting S2 components—that is covered at the top by S1. Each S1 component consists of two large domains, the N-terminal domain (NTD) and receptor-binding domain (RBD), each associated with a smaller intermediate subdomain. In virus membranes, spike glycoproteins exist in a closed form, in which the RBDs cap the top of the S2 core and are inaccessible to ACE2, and in an open form, in which one S1 component has opened to expose the RBD for ACE2 binding6,16,18,23. Recent structural studies7,24,25 on the isolated RBD of the SARS-CoV-2 spike protein in complex with ACE2 have provided a molecular description of the receptor-binding interface. Although some comparisons can be inferred from the previous cryo-electron microscopy studies on the spike protein of SARS-CoV12,18,19,23, structures of intact trimeric SARS-CoV-2 spike with bound ACE2 are needed to determine the effects of binding on the overall spike conformation.
To examine this interaction between the SARS-CoV-2 spike protein and its receptor, we mixed the ectodomains of furin-cleaved spike with the ectodomains of ACE2 and incubated them for around 60 s before plunge-freezing the mixture in liquid ethane for examination by cryo-electron microscopy. In the images that we obtained, we could resolve ten distinct species of spike and spike–ACE2 complexes (Fig. 1 and Extended Data Fig. 1), ranging from tightly closed, unbound trimers to open trimers that formed complexes with three ACE2 molecules and dissociated monomeric S1–ACE2 complexes. Of the spike trimers analysed, two thirds were bound to ACE2 (Extended Data Fig. 1). Of the unbound species, we observe good-quality particles in the closed unbound conformation, equally compact to those reported in our previous study26 and slightly more so than those described in previous reports6,16. There are also considerable numbers (16% of all trimers) of unbound particles with one erect RBD, as well as some (4%) in an intermediate conformation, a less-compact closed form, with a single disordered RBD, which have also been reported in a previous study of the furin-cleaved spike protein26.
Of the spike trimers bound to the receptor, half accommodate one ACE2 receptor. As previously reported for the SARS-CoV spike protein12,23, the ACE2-bound RBD occupies a range of tilts with respect to the long axis of the trimer (Extended Data Fig. 2a). Of the two RBDs per trimer that are not engaged with the receptor, either both are closed or one of the RBDs remains closed and one (either clockwise or anticlockwise to the bound S1 (Extended Data Fig. 1)) is in the open conformation. We were also able to identify, reconstruct and refine trimers to which two or three ACE2 receptors were bound, in successively more open structures (Fig. 1 and Extended Data Fig. 1).
Comparison of the trimers with one erect RBD that is either bound or unbound by an ACE2 receptor revealed two things. First, ACE2 binding alters the position of the open RBD by a rigid-body rotation of the domain that moves its centre of mass on average a further approximately 5.5 Å away from the trimer axis, the NTD-associated and RBD-associated subdomains of the same monomer shift around 1.9 Å and about 2.3 Å, respectively (Extended Data Fig. 2c), and at the same time the NTDs of all three S1 components move by around 1.5–3.0 Å (Extended Data Fig. 2d). Similar changes in the domain orientation are observed in the recent structure of the SARS-CoV-2 spike complex with C105 Fab27 (Extended Data Fig. 2e), which binds at the ACE2-binding site. However, the molecular basis of both of these sets of changes remains unclear. Binding of more than one ACE2 molecule does not induce any substantial further changes in the average positioning of the RBD (Extended Data Fig. 2e). Second, our data suggest that ACE2 binding favours the open conformation of the RBD. The relatively high-affinity interaction of RBD with ACE2 generates an RBD–ACE2 structure that cannot be accommodated in a closed trimer—the bound state does not have access to the closed conformation. In addition, the fact that ACE2 binding induces a more-open conformation of the spike RBD suggests that some of the binding energy is used to drive the new conformation of S1, which is then further excluded from a closed state.
The successive steps, from closed unbound trimer to the fully open, three-ACE2-bound trimer, are associated with a substantial reduction in the contact area that each S1 makes with both its neighbouring S1 monomers and with the S2 trimeric core (Extended Data Table 1). For the fully, three-ACE2-bound species, each S1 makes 1,400 Å2 less contact with both its S1 trimer neighbours and 1,300 Å2 less contact with the S2 core than in the fully closed trimer conformation; all of these rearrangements are driven by the energetics of the three ACE2-binding events. The movements of the RBD and NTD domains of S1 that are associated with the opening of the structure and stabilization of the new arrangement by ACE2 binding, as described above, leave a trimeric ring of S1 molecules that are attached to the S2 core only through contacts with its two small intermediate subdomains (Fig. 2a). Comparing the ACE2-bound, open form (the open-unbound structure is similar but of poorer local resolution) with the fully closed trimer, the RBD-associated intermediate subdomain moves about 8 Å, whereas the NTD-associated intermediate subdomain moves by 3 Å (Fig. 2a). The latter also undergoes a partial restructuring with possibly important implications for the mechanism of fusion activation of spike. In the closed form, one edge of the NTD-associated intermediate subdomain interacts with a short helix and a loop from S2 of the neighbouring monomer (Fig. 2b). Notably, two components of this interaction comprise a series of side-chain π-stacking interactions in the closed structure26: Tyr636, Phe318 and Arg634 of S1 with Tyr837 of S2; and a salt bridge formed by Asp614 of S1 with Lys854 of S2. By contrast, in the ACE2-bound form, Tyr636, Phe318 and Trp633 refold to the side of the domain further away from the symmetry axis (as viewed in Fig. 2c), leaving a channel to accommodate a new segment of α-helix that forms downstream of Asp614 from polypeptide chain that was previously disordered. As a consequence, the interactions between S1 and S2 described above for the closed form are lost in the ACE2-bound form and the segment comprising residues 827–855 of S2 becomes disordered (Fig. 2c). This part of S2 is immediately C-terminal to the putative fusion peptide of S211, the N terminus of which is defined by Arg815 at the S2′ cleavage site9,11. The opening of the ACE2-stabilized S1 therefore leads to the destabilization of the S2 structure just after the putative fusion peptide, potentially activating it for exposure in the next stages of membrane fusion. Notably, Asp614, which forms salt bridges to Lys854 of S2 in the closed form, is frequently substituted13,14,15 by a glycine residue and it has been suggested that this substitution reduces shedding of S1 (and increases the number of spike proteins on the virus surface)13. We also propose that this substitution would remove a key salt bridge, and that the unique stereochemistry available to glycine may facilitate the formation of the new segment of α-helix, which is also incompatible with the S2 interaction. Furthermore, it could lead to reduced stability of the closed form of the spike protein, which in turn would increase the likelihood of the RBDs adopting the open conformation and hence the ability of the spike protein to bind to ACE2.
The opening up, and out, from the trimer axis of the S1 domains after ACE2 binding gives rise to an unshielding of the top surface of the helix–loop–helix (approximately residues 980–990 within the HR1 region20,22,28,29) at the top of the S2 domain (Fig. 3). In the closed form, these helices and their connecting turns are tightly shielded by the RBDs; each S2 monomer is predominantly covered by its anticlockwise-related S1 trimer neighbour. In the fully open state, the S1 domains move in such a way as to generate a cavity with a diameter of 50 Å around the trimer axis that is about 65 Å deep. At the bottom of this cavity is the now solvent-exposed, central portion of HR1. For membrane fusion to occur—in comparison with other class-I fusion proteins and as described in coronavirus post-fusion structures22,28,29—the S2 component is likely to undergo a major helical rearrangement, in which the long trimer interface helix (spanning residues 990–1035) grows and extends, by incorporating the refolded turn and helix from the N-terminal portion of HR1, and projects the fusion peptide towards the host cell membrane. In this process, opening up of all three S1 monomers and their subsequent dissociation would enable the concerted helical refolding, as the cooperative displacement of the capping portions of the protein will probably be required for the extension of the helical coil, as has recently been observed for the haemagglutinin protein of influenza30. The stoichiometry of S1 subunit–ACE2 interactions required for effective cell-surface contact or for priming is not addressed by our experiments. However, as the affinity of individual monomers for ACE2 appears to be sufficient for cellular association, it may be that more than one subunit is required to be in the open form for efficient priming of these rearrangements in S2 that occur in the process of membrane fusion. It seems reasonable to propose that the likelihood of triggering the fusion conformation increases with the number of ACE2 receptors bound.
In addition to the range of species of trimeric spike described above, the largest single population of particles that we were able to identify and reconstruct represent ACE2 bound to a S1 monomer (Fig. 4). The interaction between ACE2 and the RBD, and the interaction of the latter with its associated intermediate subdomain, are very similar between the monomeric and trimer versions and with previously determined crystal and electron microscopy structures of ACE2 and RBD7,24,25. However, there are increasingly large rearrangements between the two intermediate subdomains and then with the NTD. By applying non-uniform refinement, the highest resolution was achieved for the reconstruction of the ACE2–RBD interaction (Extended Data Fig. 4), in part because of the tight interaction but also probably because of the dominant influence of this part of the structure on the alignment process. Nevertheless, it is clear that there are both increasingly large changes in the interfaces between domains on moving towards the NTD and a range of subpopulations of related but variable conformations. The high proportion of ACE2–S1 monomers, and the limited contact areas between the trimeric S1 ring interactions with S2, suggest that the fully open ACE2-bound spike complex is probably metastable.
Taken together, our structural data enable mechanistic suggestions for the early stages of SARS-CoV-2 infection of cells. The SARS-CoV-2 spike protein is produced in a compact closed form in which the helices in the S2 membrane fusion component are capped by the RBD of neighbouring monomers. After cleavage by furin between the S1 and S2 domains, the proportion of the spike trimers that is able to accommodate RBD in an open, ACE2-binding conformation increases26. Binding of the ACE2 receptor to an open RBD leads to a more-open trimer conformation. The geometry of ACE2 binding is incompatible with the RBD adopting a closed conformation and leads to our observation of several two-open-RBD conformations as well as the three-RBD-bound conformation. Successive RBD opening and ACE2 binding lead to a fully open and ACE2-bound form in which the trimeric S1 ring remains bound to the core S2 trimer by limited contacts through the intermediate subdomains of S1. This arrangement leaves the top of the S2 helices fully exposed. In the process, the interaction of the closed form of S1 with a segment of the S2 chain that precedes the putative fusion peptide region, in the open form, is lost. We suggest that in this form the S trimer is primed for the helical rearrangements of S2 that are required for fusion of the viral and host cell membranes28.
Constructs design, protein expression and purification
The ectodomains of ACE2 (19–615) and stabilized, ‘2P’ mutant (K986P and V987P) of SARS-CoV-2 spike (residues 1–1208) with intact furin-cleavage site were prepared as described in a recent study26. In brief, the proteins were expressed in Expi293F cells (Gibco), collected twice after 3–4 and 6–7 days, and purified with affinity chromatography (spike using CoNTA resin from TAKARA, ACE2 with Streptactin XT resin from IBA Lifesciences), followed by gel filtration into a buffer containing 20 mM Tris pH 8.0 and 150 mM NaCl. As previously described26, the purified spike was then incubated for 5 h with exogenous furin (New England Biolabs), after which the reaction was stopped by addition of EDTA.
Electron microscopy sample preparation and data collection
R2/2 200-mesh Quantifoil grids were glow-discharged for 30 s at 25 mA to prepare them for freezing. The furin-treated SARS-CoV-2 spike was mixed with octyl glucoside as previously described26 and, 45–60 s before ultimately plunge-freezing the grid, with concentrated ACE2 at a 1:2 final molar ratio of trimeric spike:ACE2, aiming to obtain a final concentration of spike of 0.5 mg ml−1 and octyl glucoside of 0.1%. Then, 4 μl of the obtained reaction mixture was applied on a grid pre-equilibrated at 4 °C in 100% humidity, blotted with filter paper for 4–4.5 s using Vitrobot Mark III, and plunge-frozen in liquid ethane.
Data were collected using EPU software on a Titan Krios microscope operating at 300 kV. Micrographs were collected using a Gatan K2 detector mounted on a Gatan GIF Quantum energy filter operating in zero-loss mode with a slit width of 20 eV. Exposures were 8 s, fractionated into 32 frames with an accumulated dose of 54.4 e−Å−2, with a calibrated pixel size of 1.08 Å. Images were collected at a range of defoci between 1.5 and 3.0 μm.
Electron microscopy data processing
Movies were aligned using MotionCor231 implemented in RELION32, followed by contrast transfer function (CTF) estimation using Ctffind433. Particles were picked using crYOLO34 using a manually trained model. Particles were subjected to multiple rounds of two-dimensional classification using cryoSPARC35. Classes that displayed a clear secondary structure were retained and split into subsets, which either resembled spike trimers or S1 monomers bound to ACE2. Initial models were made using the ab initio reconstruction in cryoSPARC. Different species containing trimeric spike proteins were separated by extensive three-dimensional classification in RELION as shown in Extended Data Fig. 3. Before the final refinement, particles corresponding to each of these species were subjected to Bayesian polishing in RELION36 followed by homogeneous refinement in cryoSPARC coupled to CTF refinement. The monomeric S1–ACE2 complex was classified as in Extended Data Fig. 4a and refined using non-uniform refinement in cryoSPARC coupled to CTF refinement. The final particles from the S1–ACE2 complex were subjected to an unmasked refinement in RELION to better resolve less-ordered domains, with an overall lower global resolution (Extended Data Fig. 4b, c). Local resolution was estimated using blocres37 implemented in cryoSPARC. Maps were locally filtered and globally sharpened38 in cryoSPARC (Extended Data Figs. 5, 6).
The model for the monomeric S1–ACE2 complex was based on the previously determined crystal structure (PDB: 6M0J)24, with additional parts of the RBD and intermediate domain taken from a previous structure of the closed trimer (PDB: 6ZGE)26. Models of the trimer structures were built using structures from our previous study26 for the closed trimer (PDB: 6ZGE) and the one-erect-RBD structure (PDB: 6ZGG). The RBD–ACE2 parts of the model were built using the structure from the high resolution S1–ACE2 complex from this study. Models were manually adjusted using COOT39. The models of S1–ACE2 and the one-ACE2-bound closed structure were refined and validated using PHENIX real space refine40. The other, lower resolution models were refined using NAMDINATOR41 and geometry minimization and validation in PHENIX (Extended Data Table 2). Measurements were made using Chimera42, CCP4MG43 and PISA44, with structures aligned on the large helix of S2 (residues 986–1032).
Further information on research design is available in the Nature Research Reporting Summary linked to this paper.
Maps and models have been deposited in the Electron Microscopy Data Bank (EMD) and the Protein Data Bank (PDB) with the following accession codes: EMD-11681 and PDB 7A91 (dissociated S1 domain bound to ACE2 (non-uniform refinement)); EMD-11682 and PDB 7A92 (dissociated S1 domain bound to ACE2 (unmasked refinement)); EMD-11683 and PDB 7A93 (SARS-CoV-2 spike with two RBDs erect); EMD-11684 and PDB 7A94 (SARS-CoV-2 spike with one ACE2 bound); EMD-11685 and PDB 7A95 (SARS-CoV-2 spike with one ACE2 bound and one RBD erect in clockwise direction); EMD-11686 and PDB 7A96 (SARS-CoV-2 spike with one ACE2 bound and one RBD erect in anticlockwise direction); EMD-11687 and PDB 7A97 (SARS-CoV-2 spike with two ACE2 bound); EMD-11688 and PDB 7A98 (SARS-CoV-2 spike with three ACE2 bound).
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We thank A. Nans of the Structural Biology Science Technology Platform for assistance with data collection, P. Walker and A. Purkiss of the Structural Biology Science Technology Platform and the Scientific Computing Science Technology Platform for computational support, and L. Calder, P. Cherepanov, G. Kassiotis and S. Kjaer for discussions. This work was funded by the Francis Crick Institute, which receives its core funding from Cancer Research UK (FC001078 and FC001143), the UK Medical Research Council (FC001078 and FC001143) and the Wellcome Trust (FC001078 and FC001143). P.X. is also supported by the 100 Top Talents Program of Sun Yat-sen University, the Sanming Project of Medicine in Shenzhen (SZSM201911003) and the Shenzhen Science and Technology Innovation Committee (grant no. JCYJ20190809151611269).
The authors declare no competing interests.
Peer review information Nature thanks Stephen Harrison and the other, anonymous, reviewer(s) for their contribution to the peer review of this work.
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Extended data figures and tables
The three monomers of S in each trimer are coloured in blue, rosy brown and gold with ACE2 shown in green. Relative percentages of all trimeric S particles used to calculate electron microscopy maps are shown.
a, Two three-dimensional classes, obtained by further classification of the one-ACE2-bound closed state from Fig. 1, representative of the range of motion of the RBD with bound ACE2, tilting away from the trimer axis of the spike trimer. The tilt of the RBD and ACE2 is indicated with a dashed line. b, Representative density of different obtained electron microscopy maps for residues 996–1030 of S2. Built model shown in pink, with EM density shown as a mesh. c, d, Comparison of spike structures for the open one-erect-RBD structure (purple) with the one-ACE2-bound structure (orange). c, S1 domains shown to highlight domain shifts of the RBD and RBD-associated intermediate domain. d, Outwards movements of spike domains (excluding RBDs). e, Comparison of RBD displacements of one-bound, two-bound and three-bound RBDs after binding of ACE2 to the unbound open structure of the spike protein (beige). These are compared to the RBD displacement after binding of the C105 Fab fragment27, which binds at the ACE2 interface of the RBD (PDB: 6XCM).
Classes of particles used to obtain the final spike trimer structures, unbound and in complex with ACE2, are surrounded by a box of the same colour as the final maps shown at the bottom. The global resolution, final particle number and percentage for each trimer species are shown at the bottom.
a, Classification scheme for the S1–ACE2 complex. b, c, Maps are shown of orthogonal views of the non-uniform refinement (b) and unmasked refinement (c) of the final particles. Domains are coloured as follows: green, ACE2; yellow, NTD; rosy brown, RBD; pink, RBD ganymede; blue, NTD ganymede; cream, disseminated S1 density in b.
FSC, Fourier shell correlation.
Top, orthogonal views of electron microscopy density (grey) and ribbon diagram representation of the models. Bottom, electron microscopy maps coloured by local resolution shown below.
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Benton, D.J., Wrobel, A.G., Xu, P. et al. Receptor binding and priming of the spike protein of SARS-CoV-2 for membrane fusion. Nature 588, 327–330 (2020). https://doi.org/10.1038/s41586-020-2772-0
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