Extended Data Fig. 3: EGFR surface levels are unchanged upon EXOC1 and EXOC2 knockdown in HeLa cells. | Nature

Extended Data Fig. 3: EGFR surface levels are unchanged upon EXOC1 and EXOC2 knockdown in HeLa cells.

From: Lysosome-targeting chimaeras for degradation of extracellular proteins

Extended Data Fig. 3

Cetuximab binds equally to dCas9-KRAB HeLa cells transfected with non-targeting sgRNA and cells transfected with sgRNA targeting IGF2R, EXOC1 or EXOC2, indicating no change in EGFR surface levels. Cells were subjected to live-cell flow cytometry using cetuximab followed by an anti-human Alexa Fluor-647-conjugated anti-human (anti-human 647) secondary antibody. Data are representative of two independent experiments.

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