Extended Data Fig. 6: Mixed-cell assay demonstrates that binding specificity is comparable between ctx-M6Pn and ctx. | Nature

Extended Data Fig. 6: Mixed-cell assay demonstrates that binding specificity is comparable between ctx-M6Pn and ctx.

From: Lysosome-targeting chimaeras for degradation of extracellular proteins

Extended Data Fig. 6

a, Scheme for mixed cell assay. HeLa cells were lifted and labelled with CellTracker Deep Red, then mixed in a 1:1 ratio with Jurkat cells. The mixed cell sample was stained with either 10 nM ctx or ctx-M6Pn conjugate, followed by anti-human 488, then subjected to live-cell flow cytometry. b, Cell surface CI-M6PR levels on HeLa cells (CIM6PR+EGFR+) and Jurkat cells (CIM6PR+EGFR) were measured by live-cell flow cytometry. HeLa and Jurkat cells exhibited similar levels of cell-surface CI-M6PR. c, Ctx and ctx-M6Pn exhibit equivalent binding to HeLa cells, and ctx-M6Pn exhibits minimal increased binding to Jurkat cells relative to ctx. Data are representative of two independent experiments (b) or two experimental replicates (c).

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