Extended Data Fig. 2: Surface marker profiling of structural cells under homeostatic conditions. | Nature

Extended Data Fig. 2: Surface marker profiling of structural cells under homeostatic conditions.

From: Structural cells are key regulators of organ-specific immune responses

Extended Data Fig. 2

a, Gating strategy for the flow cytometry-based validation of the structural cell sorting scheme. Identification of structural cells starts with gating for intact cells (1), single cells (2), live cells (3) and non-haematopoietic cells (4). From the resulting non-haematopoietic (CD45) cell population, potential epithelial cells (5.1) are gated for epithelial cell markers (5.2). Similarly, potential endothelial cells and fibroblasts (6.1, 6.2) are gated for endothelial cell markers (6.3) and fibroblast markers (6.4). b, Relative frequencies of potential structural cell types based on gates 5.2, 6.3 and 6.4 (from a), comparing the selected markers with alternative markers. c, Expression of the selected surface markers of structural cell types (top row) and potential alternative markers for cells gated as in Extended Data Fig. 1a. Shown are mean and s.e.m. values. Sample size (all panels): n = 3 independent biological replicates.

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