Extended Data Fig. 1: Standardized identification and purification of structural cells across 12 organs. | Nature

Extended Data Fig. 1: Standardized identification and purification of structural cells across 12 organs.

From: Structural cells are key regulators of organ-specific immune responses

Extended Data Fig. 1

a, Cell-type identification and cell-sorting scheme (top row) with representative flow cytometry plots (selected from n = 4 independent biological replicates) in one representative organ (brain) under homeostatic conditions (bottom row). b, Representative plots (selected from n = 4 independent biological replicates) for gating steps 4–6 of the standardized cell-type identification and cell-sorting scheme (a) across the 12 organs under homeostatic conditions. c, Relative frequencies of structural cell types among non-haematopoietic (CD45) cells across 12 organs, for cell suspensions obtained by standardized organ dissociation. d, Relative frequencies of structural cell types among non-haematopoietic (CD45) cells across three organs, for cell suspensions obtained by either standardized organ dissociation or organ-specific dissociation protocols. Shown are mean and s.e.m. values. Sample size: n = 4 (c) and n = 3 (d) independent biological replicates.

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